Antimicrobial, antibacterial and spore germination inhibiting activity from an avocado extract enriched in bioactive compounds

Abstract

The present disclosure relates to extracts from Persea sp. (avocado) enriched in bioactive compounds which can be used as antimicrobial, antibacterial or spore germination inhibiting agents, the process for obtaining the extracts, acetogenins and isolated molecules and methods for using the extracts enriched in bioactive compounds for providing antimicrobial, antibacterial or spore germination inhibiting effect.

1 - 17 . (canceled) 
     
     
         18 . A compound of the formula: 
       
         
           
           
               
               
           
         
         wherein:
 R is chosen from the group consisting of H and C 1-6  alkyl; 
 A is chosen from the group consisting of O and OH; 
 n is 0 or 1; and 
 m is 0 or 1. 
 
       
     
     
         19 . The compound of  claim 18 , wherein the compound has the formula: 
       
         
           
           
               
               
           
         
       
     
     
         20 . The compound of  claim 18 , wherein the compound has the formula: 
       
         
           
           
               
               
           
         
       
     
     
         21 . A pharmaceutical composition comprising:
 the compound of  claim 18  and   a pharmaceutically acceptable carrier.   
     
     
         22 . The pharmaceutical composition of  claim 21 , wherein the compound is present in a concentration of at least about 7.8 μg/ml. 
     
     
         23 . The pharmaceutical composition of  claim 21  further comprising:
 an antimicrobial substance selected from the group consisting of nitrite compounds, nisin, bacteriocins, ethyl lauroyl arginate, ethylene diaminetetraacetic acid compounds, ascorbic acid compounds, benzoic acid compounds, and combinations thereof. 
 
     
     
         24 . A food product composition comprising:
 a food product and   the compound of  claim 28 .   
     
     
         25 . The food product composition of  claim 24 , wherein the compound is present in a concentration of at least about 7.8 μg/ml. 
     
     
         26 . The food product composition of  claim 24 , wherein the food product is selected from the group consisting of fish, crustaceans, fish substitutes, crustacean substitutes, meat, meat substitutes, poultry products, vegetables, greens, sauces, emulsions, beverages, juices, wines, beers, dairy products, egg-based products, jams, jellies, grain-based products, baked goods, confectionary products, and combinations thereof. 
     
     
         27 . The food product composition of  claim 24 , wherein the food product is a ready to eat food product stored under refrigerated conditions. 
     
     
         28 . The food product composition of  claim 24  further comprising:
 an antimicrobial substance selected from the group consisting of nitrite compounds, nisin, bacteriocins, ethyl lauroyl arginate, ethylene diaminetetraacetic acid compounds, ascorbic acid compounds, benzoic acid compounds, and combinations thereof. 
 
     
     
         29 . A personal care product composition comprising:
 a personal care product and   the compound of  claim 18 .   
     
     
         30 . The personal care product composition of  claim 29 , wherein the compound is present in a concentration of at least about 7.8 μg/ml. 
     
     
         31 . The personal care product composition of  claim 29 , wherein the personal care product is selected from the group consisting of creams, gels, powders, lotions, sunscreens, lipstick, body wash, herbal extracts, formulations that support the growth of bacteria, and combinations thereof. 
     
     
         32 . The personal care product composition of  claim 29  further comprising:
 an antimicrobial substance selected from the group consisting of nitrite compounds, nisin, bacteriocins, ethyl lauroyl arginate, ethylene diaminetetraacetic acid compounds, ascorbic acid compounds, benzoic acid compounds, and combinations thereof. 
 
     
     
         33 . A cleaning product composition comprising:
 a cleaning product and   the compound of  claim 18 .   
     
     
         34 . The cleaning product composition of  claim 33 , wherein the compound is present in a concentration of at least about 7.8 μg/ml. 
     
     
         35 . The cleaning product composition of  claim 33  further comprising:
 an antimicrobial substance selected from the group consisting of nitrite compounds, nisin, bacteriocins, ethyl lauroyl arginate, ethylene diaminetetraacetic acid compounds, ascorbic acid compounds, benzoic acid compounds, and combinations thereof. 
 
     
     
         36 . A method of providing an antimicrobial or antibacterial effect on or in a product, said method comprising:
 providing an inhibitor compound of the formula:   
       
         
           
           
               
               
           
         
         wherein:
 R is chosen from the group consisting of H and C 1-6  alkyl; 
 A is chosen from the group consisting of O and OH; 
 n is 0 or 1; and 
 m is 0 or 1 
 
       
       and
 applying the inhibitor compound on or in the product to provide an antimicrobial or antibacterial effect on or in the product. 
 
     
     
         37 .- 50 . (canceled) 
     
     
         51 . A method of providing an antimicrobial or antibacterial effect on or in a subject, said method comprising:
 providing an inhibitor compound of the formula:   
       
         
           
           
               
               
           
         
         wherein:
 R is chosen from the group consisting of H and C 1-6  alkyl; 
 A is chosen from the group consisting of O and OH; 
 n is 0 or 1; and 
 m is 0 or 1 
 
       
       and
 selecting a subject having microbes or bacteria on or in the subject's body; and 
 administering to the selected subject the isolated inhibitor compound in an amount effective to provide an antimicrobial or antibacterial effect on or in the subject. 
 
     
     
         52 .- 56 . (canceled)