US2020267969A1PendingUtilityA1

Use of tree sap to preserve sperm cell lines

38
Individually held — no corporate assignee on recordPriority: Apr 27, 2015Filed: May 12, 2020Published: Aug 27, 2020
Est. expiryApr 27, 2035(~8.8 yrs left)· nominal 20-yr term from priority
Inventors:Meg A. Robinson
A01N 1/162A01N 1/125C12N 5/061A01N 1/0221A01N 1/0284
38
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Claims

Abstract

A method of cryogenically preserving sperm comprising (a) combining sperm to be cryogenically preserved and a composition that comprises (1) a cryoprotectant, comprising one or more tree saps; and (2) an extender medium to produce a sperm/medium combination; and (b) subjecting the combination to conditions that result in cryopreservation of sperm, thereby producing a cryopreserved combination that comprises cryopreserved sperm is disclosed.

Claims

exact text as granted — not AI-modified
I claim: 
     
         1 . A method of cryogenically preserving sperm comprising:
 a. combining sperm to be cryogenically preserved and a composition that comprises (1) a cryoprotectant, comprising one or more tree saps; and (2) an extender medium to produce a sperm/medium combination; and   b. subjecting the combination to conditions that result in cryopreservation of sperm, thereby producing a cryopreserved combination that comprises cryopreserved sperm,   wherein the one or more tree saps is derived from cold-hardy trees.   
     
     
         2 . The method of  claim 1  wherein the cryopreserved sperm of step (b) demonstrates survival greater than 50% after thawing. 
     
     
         3 . The method of  claim 1  wherein the cryopreserved sperm of step (b) demonstrates motility greater than 30% after thawing. 
     
     
         4 . The method of  claim 1  wherein the one of more tree saps is the only cryoprotectant. 
     
     
         5 . The method of  claim 1  wherein an additional cryoprotectant is added. 
     
     
         6 . The method of  claim 1  wherein the sperm is avian sperm. 
     
     
         7 . The method of  claim 6  wherein the sperm is derived from the Northern goshawk ( Accipiter gentilis ). 
     
     
         8 . The method of  claim 1  wherein the sperm is derived from a non-human mammal. 
     
     
         9 . The method of  claim 8  wherein the sperm is derived from an animal type selected from the group consisting of canine, avian, cattle, porcine and equine. 
     
     
         10 . The method of  claim 1  wherein the one or more tree saps is a first run sap. 
     
     
         11 . The method of  claim 1  wherein the extender medium does not contain fructose. 
     
     
         12 . The method of  claim 1  wherein the method comprises the additional step of subjecting the combination to a temperature between −80° C. and −198° C. for a period of at least one day. 
     
     
         13 . The cryopreserved combination resulting from the method of  claim 1 . 
     
     
         14 . The cryopreserved combination resulting from the method of  claim 2 . 
     
     
         15 . The cryopreserved combination resulting from the method of  claim 3 . 
     
     
         16 . A method of fertilizing an egg cell comprising the step of thawing a cryopreserved combination produced by the method of  claim 1 , and introducing the combination to an unfertilized egg cell, wherein the egg cell becomes fertilized. 
     
     
         17 . The method of  claim 16 , wherein the egg cell is an avian egg. 
     
     
         18 . The method of  claim 16 , wherein the egg cell is a mammalian egg. 
     
     
         19 . A composition comprising tree sap, semen, and extender medium, wherein the tree sap is a cold-hardy tree sap. 
     
     
         20 . The composition of  claim 19 , wherein the sap is at least 50% by volume of the composition.

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