Dental pretreatment material and dental tissue regeneration kit

Abstract

Provided is a dental pretreatment material for dental tissue regeneration by use of dental pulp stem cells, particularly a dental pretreatment material effectively enabling dental tissue regeneration even by use of dental pulp stem cells of middle-aged or older individuals. The dental pretreatment material is characterized by comprising a serine protease, specifically trypsin. The dental pretreatment material comprising trypsin is used as an injection into a root canal before a root canal filling material comprising dental pulp stem cells and an extracellular matrix is inserted into the root canal as an attempt to regenerate a dental pulp and a dentin. The root canal filling material includes an ALK5 inhibitor, a CCR3 antagonist, or a CCL11 neutralizing antibody.

1 . A dental pretreatment material for dental tissue regeneration, the dental pretreatment material comprising a serine protease. 
     
     
         2 . The dental pretreatment material of  claim 1 , wherein the serine protease is a chymotrypsin-like serine protease. 
     
     
         3 . The dental pretreatment material of  claim 2 , wherein the chymotrypsin-like serine protease is trypsin. 
     
     
         4 . The dental pretreatment material of  claim 1 , further comprising nano-bubbles. 
     
     
         5 . The dental pretreatment material of  claim 4 , wherein the nano-bubbles have a diameter ranging from 10 nm to 500 nm. 
     
     
         6 . The dental pretreatment material of  claim 1 , wherein the dental tissue regeneration is carried out by use of autologous or allogeneic dental pulp stem cells in an individual of middle or advanced age.