Novel lactococcus lactis subspecies lactis isolate wflu-12 and use thereof

Abstract

The present invention provides a Lactococcus lactis subspecies lactis isolate WFLU-12 with the accession number of KCTC 13180BP, and a use thereof.

1 . A probiotic composition comprising a  Lactococcus lactis  subspecies  lactis  isolate WFLU-12 with the accession number KCTC13180BP, a culture thereof, a lysate thereof or an extract thereof as an active ingredient. 
     
     
         2 . The probiotic composition of  claim 1 , wherein the isolate shows resistance to a temperature of at least 4° C., a pH of 2-10, and a bile acid. 
     
     
         3 . The probiotic composition of  claim 1 , wherein the isolate has an antibacterial activity against at least one selected from the group consisting of a Gram-negative bacterium of  Vibrio anguillarum, V. ichthyoenteri, Aeromonas salmonicida, Edwardsiella tarda , or a Gram-positive bacterium of  Streptococcus iniae, S. parauberis.    
     
     
         4 . (canceled) 
     
     
         5 . The probiotic composition of  claim 1 , wherein the probiotic composition presents at least one effect selected from enhancing innate immune response of fish, increasing body weight, increasing body length, enhancing body circulating metabolite, maintaining homeostasis of metabolite, increasing sulfur-containing amino acid level in body, increasing taurine level in intestine, enhancing citrulline level in body, and enhancing vitamin level in intestine. 
     
     
         6 . An antibacterial composition comprising a  Lactococcus lactis  subspecies  lactis  isolate WFLU-12 with the accession number KCTC13180BP, a culture thereof, a lysate thereof or an extract thereof as an active ingredient. 
     
     
         7 . A feed additive for fish farming, comprising the probiotic composition of  claim 1  or the antibacterial composition of  claim 6  as an active ingredient. 
     
     
         8 - 10 . (canceled)