Methods for Enriching Pluripotent Stem Cell-Derived Cardiomyocyte Progenitor Cells and Cardiomyocyte Cells based on SIRPA Expression

Abstract

The present invention relates to in vitro methods of enriching populations of human pluripotent stem cells that are induced to differentiate to cardiomyocyte progenitor cells and cardiomyocyte cells. The cell populations can be enriched by isolating cells that express SIRPA. The invention also related to in vitro-enriched populations of cardiomyocyte cells and cardiomyocyte progenitor cells obtained from populations of pluripotent stem.

1 - 29 . (canceled) 
     
     
         30 . A method of enriching a population of cells for cardiomyocyte cells and cardiomyocyte progenitor cells comprising:
 (a) providing the population of cells from which cardiomyocyte cells and cardiomyocyte progenitor cells are to be isolated; and   (b) depleting from the population, cells expressing at least one of CD90, CD31, CD140B and CD49A;   wherein the population of cells comprises a population of human pluripotent stem cells induced to differentiate into cardiomyocyte cells, and cardiomyocyte progenitor cells, and noncardiomyocytes.   
     
     
         31 . The method of  claim 30 , wherein the step of depleting from the population cells expressing at least one of CD90, CD31, CD140B and CD49A comprises a step of sorting the cells with one or more antibodies, wherein the one or more antibodies is selected from the group consisting of an anti-CD90 antibody, an anti-CD31 antibody, an anti-CD140B antibody, and an anti-CD49A antibody. 
     
     
         32 . The method of  claim 31 , wherein the one or more antibodies comprises an anti-CD90 antibody. 
     
     
         33 . The method of  claim 31 , wherein the one or more antibodies comprises an anti-CD31 antibody. 
     
     
         34 . The method of  claim 31 , wherein the one or more antibodies comprises an anti-CD140B antibody. 
     
     
         35 . The method of  claim 31 , wherein the one or more antibodies comprises an anti-CD49A antibody. 
     
     
         36 . The method of  claim 31 , wherein the method comprises a step of sorting the cells with an anti-CD90 antibody, an anti-CD31 antibody, an anti-CD140B antibody and an anti-CD49A antibody.