Factor ix polypeptide mutant, its uses and a method for its production

Abstract

Disclosed are a modified FIX (Factor IX) polypeptide comprising a leucine, cysteine, aspartic acid, glutamic acid, histidine, lysine, asparagine, glutamine or tyrosine in position 338; pharmaceutical preparations containing said modified FIX polypeptide; a nucleotide sequence coding for the modified FIX polypeptide; and a method for producing the modified FIX polypeptide.

What is claimed is: 
     
         1 . A modified FIX (factor IX) polypeptide comprising: an amino acid chosen from a group consisting of: leucine, cysteine, aspartic acid, glutamic acid, histidine, lysine, asparagine, glutamine, and tyrosine at a position corresponding to position 338. 
     
     
         2 . A nucleic acid molecule encoding a modified FIX polypeptide according to  claim 1 . 
     
     
         3 . The modified FIX polypeptide according to  claim 1  for use as a medicament. 
     
     
         4 . A method to perform replacement therapy, the method comprising administering to an individual in need thereof the modified FIX polypeptide according to  claim 1 . 
     
     
         5 . A pharmaceutical composition comprising a modified recombinant FIX polypeptide according to  claim 1 , and at least one pharmaceutically acceptable excipient. 
     
     
         6 . A method for producing a modified FIX polypeptide according to  claim 1 , whereby the modified FIX polypeptide is expressed by means of a nucleic acid molecule comprising a nucleotide sequence encoding the modified FIX polypeptide.