Simple fixation and stablisation

Abstract

It has surprisingly been discovered that it is possible to stabilise biomolecules such as RNA, DNA and proteins in biological samples such as cells, tissues, biopsies and blood using deep eutectic solvents (DES). It has also been discovered that DES mixtures can be used to fix and preserve cell morphology in biological samples such as tissue blocks, cancer biopsies and whole blood. This invention describes methods to stabilise and preserve biomolecules, whole cells, tissues, blood and biological samples using DES mixtures.

1 - 159 . (canceled) 
     
     
         160 . A composition comprising a eutectic solvent, wherein said eutectic solvent comprises a first component and a second component; wherein said first component is trimethylglycine and said second component is trifluoroacetamide; wherein said first component and said second component are in a molar ratio of between about 1:1.5 and about 1:2.5; and wherein said first component and said second component together comprise at least about 88% by weight of the composition. 
     
     
         161 . The composition of  claim 160 , which further comprises a sample comprising one or more of a biomolecule, RNA, DNA, protein, phosphoprotein, virus, cell, tissue, solid tissue, plasma, serum, brain, cerebrospinal fluid, whole blood, or whole blood comprising a circulating tumor cell. 
     
     
         162 . The composition of  claim 160 , which further comprises a sample comprising one or more of viruses, influenza, human papillomavirus, human immunodeficiency virus, hepatitis A virus, hepatitis C virus, hepatitis E virus, foot and mouth disease virus, severe acute respiratory syndrome (SARS) virus, West Nile virus, Ebola virus, yellow fever virus, dengue fever virus, bacteria,  Escherichia coli, Staphylococcus  spp.,  Streptococcus  spp.,  Mycobacterium  spp.,  Pseudomonas  spp., bacteria that cause shigella, diphtheria, tetanus, syphilis, chlamydia, legionella, listeria, or leprosy, parasites,  Leishmania  spp.,  Trypanosoma  spp., or  Plasmodium  spp. 
     
     
         163 . The composition of  claim 161 , wherein said eutectic solvent prevents degradation of a biomolecule in said sample. 
     
     
         164 . The composition of  claim 160 , wherein the molar ratio of said first component to said second component is about 1:2. 
     
     
         165 . The composition of  claim 160 , wherein the eutectic solvent has a pH between 5 and b  7 . 5 . 
     
     
         166 . The composition of  claim 160 , wherein the eutectic solvent further comprises zinc sulfate, a desiccant, sodium dodecyl sulfate, sodium benzoate, N-methyl pyrrolidone, N-ethyl pyrrolidone, 1-methylimidazole, 1-benzylimidazole, tetramethylurea, 4-Formyl morpholine, water, and/or acetic acid. 
     
     
         167 . The composition of  claim 166 , wherein said desiccant comprises a silica gel and/or a molecular sieve. 
     
     
         168 . The composition of  claim 166 , wherein said zinc sulfate is present in the eutectic solvent in an amount in the range 0.01% to 1.5% by weight of the eutectic solvent. 
     
     
         169 . A composition comprising a eutectic solvent, wherein said eutectic solvent comprises a first component and a second component; wherein said first component is trimethylglycine and said second component is trifluoroacetamide; wherein said first component and said second component are in a molar ratio of between about 1:1.8 and about 1:2.2; and wherein said first component and said second component together comprise at least about 88% by weight of the composition. 
     
     
         170 . The composition of  claim 169 , which further comprises a sample comprising one or more of a biomolecule, RNA, DNA, protein, phosphoprotein, virus, cell, tissue, solid tissue, plasma, serum, brain, cerebrospinal fluid, whole blood, or whole blood comprising a circulating tumor cell. 
     
     
         171 . The composition of  claim 169 , which further comprises a sample comprising one or more of viruses, influenza, human papillomavirus, human immunodeficiency virus, hepatitis A virus, hepatitis C virus, hepatitis E virus, foot and mouth disease virus, severe acute respiratory syndrome (SARS) virus, West Nile virus, Ebola virus, yellow fever virus, dengue fever virus, bacteria, Escherichia coli, Staphylococcus  spp.,  Streptococcus  spp.,  Mycobacterium  spp.,  Pseudomonas  spp., bacteria that cause shigella, diphtheria, tetanus, syphilis, chlamydia, legionella, listeria, or leprosy, parasites,  Leishmania  spp.,  Trypanosoma  spp., or  Plasmodium  spp. 
     
     
         172 . The composition of  claim 170 , wherein said eutectic solvent prevents degradation of a biomolecule in said sample. 
     
     
         173 . The composition of  claim 169 , wherein the molar ratio of said first component to said second component is 1:2. 
     
     
         174 . The composition of  claim 169 , wherein the eutectic solvent has a pH between 5 and 7.5. 
     
     
         175 . The composition of  claim 169 , wherein the eutectic solvent further comprises zinc sulfate, a desiccant, sodium dodecyl sulfate, sodium benzoate, N-methyl pyrrolidone, N-ethyl pyrrolidone, 1-methylimidazole, 1-benzylimidazole, tetramethylurea, 4-Formyl morpholine, water, and/or acetic acid. 
     
     
         176 . The composition of  claim 175 , wherein said desiccant comprises a silica gel and/or a molecular sieve. 
     
     
         177 . The composition of  claim 175 , wherein said zinc sulfate is present in the eutectic solvent in an amount in the range 0.01% to 1.5% by weight of the eutectic solvent. 
     
     
         178 . A composition comprising a eutectic solvent, wherein said eutectic solvent comprises a first component and a second component; wherein said first component is trimethylglycine and said second component is trifluoroacetamide; wherein said first component and said second component are in a molar ratio of between about 1:1.5 and about 1:2.5; and wherein said first component and said second component together comprise at least about 88% by weight of the composition, which further comprises a sample comprising one or more coronaviruses. 
     
     
         179 . The composition of  claim 178 , wherein said coronavirus comprises a severe acute respiratory syndrome (SARS) virus.