Photosensitising composition and its uses

Abstract

The present invention provides a photosensitising composition comprising a mixture of at least one oxygen carrier, at least one oxidising agent and at least one surfactant, and its uses. The ratio of the at least one oxygen carrier to the at least one oxidising agent to the at least one surfactant may be in the range of 50:40:10 to 80:19.8:0.2. The photosensitising composition may be used for treating and/or preventing conditions caused by microorganisms.

1 . A photosensitising composition comprising a mixture of: at least one oxygen carrier at least one oxidising agent; and at least one surfactant, wherein the ratio of the volume of the at least one oxygen carrier to the at least one oxidising agent to the at least one surfactant is in the range of 50:40:10 to 80:19.8:0.2. 
     
     
         2 . The photosensitising composition according to  claim 1 , wherein the ratio of the volume of the at least one oxygen carrier to the at least one oxidising agent to the at least one surfactant is from 60:39:1 to 76:23.6:0.4. 
     
     
         3 . The photosensitising composition according to  claim 1  or  claim 2 , wherein the ratio of the volume of the at least one oxygen carrier to the at least one oxidising agent to the at least one surfactant is 75:24.5:0.5. 
     
     
         4 . The photosensitising composition according to any one of the preceding claims, wherein the composition is in the form of an emulsion. 
     
     
         5 . The photosensitising composition according to any one of the preceding claims, wherein the at least one oxygen carrier is selected from the group consisting of: perfluorodecahydro naphthalene, perfluorodecalin, perfluorohexane, octafluoropropane, perfluorobutane, perfluorooctane, perfluoromethyldecalin and O 2 IrCl(CO)(P[C 6 H 5 ] 3 ) 2 . 
     
     
         6 . The photosensitising composition according to any one of the preceding claims, wherein the at least one oxidising agent is selected from the group consisting of: hydrogen peroxide, dilute sodium hypochlorite, DMSO and chlorine dioxide. 
     
     
         7 . The photosensitising composition according to any one of the preceding claims, wherein the at least one surfactant is selected from the group consisting of: mineral oil, glycerol, polyethylene glycol, non-ionic detergent, polypropylene glycol and SDS. 
     
     
         8 . The photosensitising composition according to  claim 7 , wherein the non-ionic detergent is Triton X. 
     
     
         9 . The photosensitising composition according to  claim 7  or  claim 8 , wherein the non-ionic detergent is Triton X-100. 
     
     
         10 . The photosensitising composition according to any one of the preceding claims, wherein the composition comprises a mixture of perfluorodecahydro naphthalene, hydrogen peroxide and Triton X. 
     
     
         11 . The photosensitising composition according to  claim 10 , wherein the wherein the ratio of the volume of perfluorodecahydro naphthalene to hydrogen peroxide to Triton X is 75:24.5:0.5. 
     
     
         12 . The photosensitising composition according to any one of the preceding claims, wherein the composition further comprises at least one photosensitising compound. 
     
     
         13 . The photosensitising composition according to  claim 12 , wherein the at least one photosensitising compound is selected from the group consisting of: toluidene blue, methylene blue, arianor steel blue, tryptan blue, crystal violet, azure blue cert, azure B chloride, azure 2, azure A chloride, azure B tetrafluoroborate, thionin, azure A eosinate, azure B eosinate, azure mix sicc, azure II eosinate, haematoporphyrin HCl, haematoporphyrin ester, aluminium disulphonated phthalocyanine, chlorins, photoactive fullerenes (e.g. C16-b), aminolevulinic acid (ALA), bacteriochlorins, phthalocyanines, pheophorbides, purpurins, naphthalocyanines, indocyanine green, or mixtures thereof. 
     
     
         14 . The photosensitising composition according to  claim 12  or  claim 13 , wherein the at least one photosensitising compound is methylene blue. 
     
     
         15 . The photosensitising composition according to any one of the preceding claims, wherein the composition further comprises a mixture of polyethylene glycol, ethanol and water. 
     
     
         16 . The photosensitising composition according to  claim 15 , wherein the polyethylene glycol is glycerol. 
     
     
         17 . The composition according to any one of the preceding claims, wherein the composition further comprises a pharmaceutically acceptable excipient and/or carrier. 
     
     
         18 . The composition according to any one of the preceding claims, wherein the composition is formulated for use in the treatment and/or prevention of conditions caused by microorganisms. 
     
     
         19 . The composition according to any one of the preceding claims, wherein the composition is formulated for use in the treatment and/or prevention of periodontal and/or halitosis conditions. 
     
     
         20 . The composition according to any one of the preceding claims, wherein the composition is formulated for topical administration. 
     
     
         21 . The composition according to any one of the preceding claims, wherein the composition is formulated for administration by injection. 
     
     
         22 . The composition according to any one of the preceding claims, wherein the composition is formulated as an oral rinse, a mouthwash and/or an atomizing spray. 
     
     
         23 . A composition according to any one of the preceding claims for use in medicine. 
     
     
         24 . Use of a photosensitising composition according to any one of  claims 1  to  22  in the manufacture of a medicament for treating and/or preventing conditions caused by microorganisms in a subject, the treatment and/or prevention comprising the steps of:
 a) administering the photosensitising composition; and 
 b) Irradiating the area to which the composition is administered with light at a wavelength absorbed by a photosensitising compound. 
 
     
     
         25 . The use according to  claim 24 , wherein the medicament is for treating and/or preventing conditions caused by microorganisms in the oral cavity of a subject. 
     
     
         26 . The use according to  claim 24  or  claim 25 , wherein the medicament is for treating and/or preventing periodontal and/or halitosis conditions. 
     
     
         27 . The use according to any one of  claims 24  to  26 , wherein the conditions consist of gingivitis, periodontitis dental caries, root caries, root canal infection and apical periodontitis. 
     
     
         28 . The use according to any one of  claims 24  to  27 , wherein the composition in step a) is administered topically or by injection. 
     
     
         29 . The use according to any one of  claims 24  to  28 , wherein the irradiation of step b) is carried out for a time period of 10 seconds to 30 minutes. 
     
     
         30 . The use according to any one of  claims 24  to  29 , wherein the dose of light used in step b) ranges from 10 J/cm 2  to 200 J/cm 2 . 
     
     
         31 . The use according to any one of  claims 24  to  30 , wherein the light used in step b) has a wavelength ranging from about 400 nm to about 1400 nm. 
     
     
         32 . A method of treating and/or preventing conditions caused by microorganisms in a subject, wherein the method comprises the steps of:
 a) administering a photosensitising composition according to any one of  claims 1  to  22 ; and   b) irradiating the area to which the composition is administered with light at a wavelength absorbed by a photosensitising compound.   
     
     
         33 . The method according to  claim 32 , wherein the method is for treating and/or preventing conditions caused by microorganisms in the oral cavity of a subject. 
     
     
         34 . The method according to  claim 32  or  claim 33 , wherein the method is for treating and/or preventing periodontal and/or halitosis conditions. 
     
     
         35 . The method according to any one of  claims 32  to  34 , wherein the conditions consist of gingivitis, periodontitis dental caries, root caries, root canal infection and apical periodontitis. 
     
     
         36 . The method according to any one of  claims 32  to  35 , wherein the composition in step a) is administered topically or by injection. 
     
     
         37 . The method according to any one of  claims 32  to  36 , wherein the irradiation of step b) is carried out for a time period of 10 seconds to 30 minutes. 
     
     
         38 . The method according to any one of  claims 32  to  37 , wherein the dose of light used in step b) ranges from 10 J/cm 2  to 200 J/cm 2 . 
     
     
         39 . The method according to any one of  claims 32  to  38 , wherein the light used in step b) has a wavelength ranging from about 400 nm to about 1400 nm. 
     
     
         40 . A kit for treating and/or preventing conditions caused by microorganisms in a subject, the kit comprising a photosensitising composition according to any one of  claims 1  to  22 , disposed in at least one suitable container. 
     
     
         41 . The kit according to  claim 40 , wherein the kit is for treating and/or preventing conditions caused by microorganisms in the oral cavity of a subject. 
     
     
         42 . The kit according to  claim 40  or  claim 41 , wherein the kit is for treating and/or preventing periodontal and/or halitosis conditions. 
     
     
         43 . The kit according to any one of  claims 40  to  42 , wherein the kit further comprises at least one light emitting device capable of emitting light at a wavelength absorbed by a photosensitising compound. 
     
     
         44 . The kit according to any one of  claims 40  to  43 , wherein the photosensitising composition comprises at least one photosensitising compound. 
     
     
         45 . A method of preparing the composition according to any one of  claims 1  to  22 , comprising the steps of:
 a) preparing a mixture of at least one oxygen carrier; at least one oxidising agent; and at least one surfactant, wherein the ratio of the volume of the at least one oxygen carrier to the at least one oxidising agent to the at least one surfactant in the mixture is in the range of 50:40:10 to 80:19.8:0.2. 
 
     
     
         46 . The method according to  claim 45 , wherein the mixture obtained in step a) is prepared by sonicating or vortexing the at least one oxygen carrier, at least one oxidising agent, and at least one surfactant. 
     
     
         47 . The method according to  claim 45  or  claim 46 , wherein the method further comprises the step of adding at least one photosensitising compound to the mixture of step a). 
     
     
         48 . The method according to any one of  claims 45  to  47 , wherein the method further comprises the step of adding polyethylene glycol, ethanol and water. 
     
     
         49 . The method according to  claim 48 , wherein the polyethylene glycol is glycerol. 
     
     
         50 . The method according to  claim 48  or  claim 49 , wherein the ratio of the volume of the polyethylene glycol to ethanol to water is 30:20:50.