Adult stem cell line introduced with hepatocyte growth factor gene and neurogenic transcription factor gene with basic helix-loop-helix motif and uses thereof

Abstract

The present invention relates to an adult stem cell line introduced with an HGF gene and a neurogenic transcription factor gene of a bHLH family, a preparation method of the adult stem cell line, a composition for the prevention or treatment of neurological diseases comprising the adult stem cell line, and a method for treating neurological diseases comprising the step of administering the composition to a subject having neurological diseases or suspected of having neurological diseases. The adult stem cells according to the present invention, which are introduced with an HGF gene and a neurogenic transcription factor gene of a bHLH family, can be used to overcome chronic impairment caused by cell death following stroke. Thus, the adult stem cells can be developed as a novel therapeutic agent or widely used in clinical trial and research for cell replacement therapy and gene therapy that are applicable to neurological diseases including Parkinson's disease, Alzheimer disease, and spinal cord injury as well as stroke.

1 .- 14 . (canceled) 
     
     
         15 . A mesenchymal stem cell modified by introducing a gene encoding a hepatocyte growth factor (HGF) and a gene encoding neurogenin 1 into the mesenchymal stem cell. 
     
     
         16 . The mesenchymal stem cell of  claim 15 , derived from one or more tissues selected from the group consisting of bone marrow, blood, umbilical cord blood, adipose tissue, liver, skin, gastrointestinal tract, placenta, and uterus. 
     
     
         17 . The mesenchymal stem cell of  claim 15 , wherein the neurogenin 1 has a nucleic acid sequence of SEQ ID NO 2. 
     
     
         18 . The mesenchymal stem cell of  claim 15 , wherein the HGF has a nucleic acid sequence of SEQ ID NO 1. 
     
     
         19 . The mesenchymal stem cell of  claim 15 , wherein the HGF gene is encoded on an extrachromosomal element that is an adenoviral vector. 
     
     
         20 . A method of preparing a culture of modified mesenchymal stem cells, comprising:
 introducing a gene encoding HGF having a nucleic acid sequence of SEQ ID NO 1 and a gene encoding neurogenin 1 having a nucleic acid sequence of SEQ ID NO 2 into a cultured mesenchymal stem cell to produce the modified mesenchymal stem cell;   selecting the modified mesenchymal stem cell that is introduced with both genes coding HGF and neurogenin 1; and   culturing the selected modified mesenchymal stem cell.   
     
     
         21 . The method of  claim 20 , wherein introducing the gene encoding HGF and introducing the gene encoding neurogenin 1 are performed sequentially, simultaneously, or in reverse order. 
     
     
         22 . The method of  claim 20 , wherein the mesenchymal stem cell is a stem cell derived from one or more tissues selected from the group consisting of bone marrow, blood, umbilical cord blood, adipose tissue and placenta. 
     
     
         23 . The method of  claim 20 , comprising introducing the gene encoding HGF into the mesenchymal stem cell by an adenoviral vector.