Process for preparing metabolized conditioned growth media

Abstract

Compositions comprising metabolized conditioned growth medium and/or metabolized cell extract and methods of use are described. The metabolized conditioned growth medium and metabolized cell extract compositions may be formulated with an acceptable carrier into injectable or topical formulations, for example, as a cream, lotion or gel, and may be used in cosmeceutical or pharmaceutical applications. The metabolized conditioned growth medium and metabolized cell extract may also be further processed to concentrate or reduce one or more factors or components contained within the metabolized conditioned growth medium or metabolized cell extract. The growth medium may be conditioned by any eukaryotic cell. The metabolized conditioned growth medium and metabolized cell extract may be used to prevent or treat a condition, for example, a skin condition.

What is claimed is: 
     
         1 . A topical composition comprising:
 metabolized cell extract; and   a suitable vehicle,   wherein the topical composition is obtainable by a process comprising the steps:
 (a) providing a fibroblast cell extract; 
 (b) culturing yeast cells; 
 (c) exposing the yeast cells to the fibroblast cell extract; 
 (d) culturing the yeast cells to metabolize at least a portion of the fibroblast cell extract; 
 (e) collecting the metabolized fibroblast cell extract; and 
 (f) combining the metabolized fibroblast cell extract with the suitable vehicle. 
   
     
     
         2 . The topical composition according to  claim 1 , further comprising at least one of water, surfactant, emulsifier, conditioner, emollient, wax, oil, polymer, thickener, fixative, colorant, nutraceutical, cosmeceutical, therapeutic, pharmaceutical, antifungal, antimicrobial, steroidal hormone, antidandruff agent, anti-acne component, sunscreen, and preservative. 
     
     
         3 . The topical composition according to any one of  claim 1  or  2 , wherein the topical composition is an ointment, a cream, a hydrogel, or a lotion. 
     
     
         4 . The topical composition according to  claim 1 , wherein the yeast is selected from a family selected from:  Neurospora, Ceratostomella, Claviceps, Xylaria, Rosellinia, Helotium, Sclerotinia, Tulostoma, Rhizopogon, Truncocolumella, Mucor, Rhizopus, Entomophthora, Dictostylium, Blastocladia, Synchytrium, Saprolegnia, Peronospora, Albugo, Pythium, Phytophthora, Plasmodiophora, Tuber, Hydnum, Lecanora, Roccella, Pertusaria, Usnea, Evernia, Ramalina, Alectoria, Cladonia, Parmelia, Cetraria, Agaricus, Cantharellus, Omphalotus, Coprinus, Lactarius, Marasmius, Pleurotus, Pholiota, Russula, Lactarius, Stropharia, Entoloma, Lepiotaceae, Corticium, Pellicularia, Tricholoma, Volvaria, Clitocybe, Flammulina, Saccharomyces, Schizosaccharomyces, Saccharomycetaceae, Eurotium, Aspergillus, Thielavia, Peziza, Plectania, Morchella, Wynnea, Helvella, Gyromitra, Phallales, Dictyophera, Mutinus, Clathrus, Pseudocolus, Lycoperdon, Calvatia, Geastrum, Radiigera, Astreus, Nidularia, Gastrocybe, Macowanites, Gastroboletus, Albatrellus, Neolentinus, Nigroporus, Oligoporus, Polyporus, Fistulina, Fomes, Boletus, Fuscoboletinus, Leccinum, Phylloporus, Suillus, Strobilomyces, Boletellus, Tremella, Auricularia, Dacrymyces, Melampsora, Cronartium, Puccinia, Gymnosporangium, Tilletia, Urocystis, Septobasidium, Hygrocybe, Hygrophorus, Hygrotrama, Neohygrophorus, Cortinarius, Gymnopilus, Trichophyton, Microsporum, Monilia, Candida, Cercosporella, Penicillium, Blastomyces, Cercospora, Ustilaginoidea, Tuber cularia, Fusarium, Rhizoctinia, Ozonium, Sclerotium, Geoglossum , and  Armillaria.    
     
     
         5 . The topical composition according to  claim 4 , wherein the yeast is selected from the family Saccharomycetaceae. 
     
     
         6 . The topical composition according to  claim 5 , wherein the yeast is selected from the genus  Pichia.    
     
     
         7 . The topical composition according to  claim 6 , wherein the yeast is selected from the species  pastoris.    
     
     
         8 . The topical composition according to  claim 1  for use in a method of therapy. 
     
     
         9 . The topical composition according to  claim 1  for use in a method of treating wounds. 
     
     
         10 . Use of the topical composition according to  claim 1  for the cosmetic treatment of a skin condition.