Calibration fluid comprising pyrogallol for the calibration of blood gas, electrolyte, and/or metabolite instrument oxygen sensor(s)

Abstract

Compositions, devices, kits, and methods for calibrating at least one oxygen sensor in a blood gas, electrolyte, and/or metabolite instrument utilizing a calibration fluid comprising a pyrogallol oxygen scavenger.

What is claimed is: 
     
         1 . A calibration fluid for calibrating at least one pO 2  sensor of a blood gas, electrolyte, and/or metabolite instrument, comprising:
 at least one calibration and/or quality control reagent comprising oxygen; and   a reagent comprising a pyrogallol oxygen scavenger,   wherein a specific amount of the reagent comprising a pyrogallol oxygen scavenger is combined with the at least one calibration and/or quality control reagent comprising oxygen such that the pyrogallol oxygen scavenger complexes oxygen present in the at least one calibration and/or quality control reagent comprising oxygen to provide a desired oxygen concentration in the calibration fluid, and wherein the desired oxygen concentration is measured via at least one electrode,   wherein the at least one calibration and/or quality control reagent comprising oxygen and the reagent comprising a pyrogallol oxygen scavenger are in aqueous solution, wherein the at least one calibration and/or quality control reagent comprising oxygen and the reagent comprising a pyrogallol oxygen scavenger are disposed in a substantially air tight environment until use thereof.   
     
     
         2 . The calibration fluid of  claim 1 , wherein the pyrogallol oxygen scavenger comprises a concentration from about 0.05% to about 1% of the calibration fluid. 
     
     
         3 . The calibration fluid of  claim 1 , wherein the specific amount of the reagent comprising a pyrogallol oxygen scavenger is from about 0.1 milliliter to about 2 milliliters. 
     
     
         4 . The calibration fluid of  claim 1 , wherein the electrode is a bar metal electrode. 
     
     
         5 . The calibration fluid of  claim 1 , wherein the desired oxygen concentration comprises a partial pressure of oxygen of about zero millimeters of mercury (mmHg). 
     
     
         6 . A method of calibrating at least one pO 2  sensor of a blood gas, electrolyte, and/or metabolite instrument, the method comprising the steps of:
 exposing at least one calibration and/or quality control reagent comprising oxygen to a specific concentration of a reagent comprising a pyrogallol oxygen scavenger to thereby form a calibration fluid, such exposure occurring immediately prior to the use of the calibration fluid, wherein the exposure is performed at a specific reaction temperature and for a specific amount of time, whereby the calibration fluid is thereby provided with a desired oxygen concentration based upon the concentration of the pyrogallol oxygen scavenger and the time and temperature of the exposure, and wherein the desired oxygen concentration is measured via at least one electrode of a sensor array, the sensor array comprising the at least one electrode and at least one pO 2  sensor; and   contacting the calibration fluid having the desired oxygen concentration with the at least one pO 2  sensor of the sensor array for calibration of the at least one pO 2  sensor,   wherein the at least one calibration and/or quality control reagent comprising oxygen and the reagent comprising a pyrogallol oxygen scavenger are in aqueous solution,   wherein the at least one calibration and/or quality control reagent comprising oxygen and the reagent comprising a pyrogallol oxygen scavenger are disposed in a substantially air tight environment until use thereof.   
     
     
         7 . The method of  claim 6 , wherein the pyrogallol oxygen scavenger comprises from about 0.05% to about 1% of the calibration fluid. 
     
     
         8 . The method of  claim 6 , wherein the specific reaction temperature if from about 20° C. to about 26° C. 
     
     
         9 . The method of  claim 6 , wherein the specific reaction time is from about 0.01 second to about 60 seconds. 
     
     
         10 . The method of  claim 6 , wherein the desired oxygen concentration comprises a partial pressure of oxygen of about zero millimeters of mercury (mmHg). 
     
     
         11 . A kit for calibrating at least one pO 2  sensor of a blood gas, electrolyte, and/or metabolite instrument, the kit comprising:
 a calibration fluid, comprising:
 at least one calibration and/or quality control reagent comprising oxygen; and 
 a reagent comprising a pyrogallol oxygen scavenger; and 
   a sensor array comprising at least one electrode capable of measuring a desired concentration of oxygen in the calibration fluid,   wherein, when calibration of the at least one pO 2  sensor is desired, a specific amount of the reagent comprising a pyrogallol scavenger is combined with the at least one calibration and/or quality control reagent comprising oxygen such that the pyrogallol oxygen scavenger complexes oxygen present in the at least one calibration and/or quality control reagent to provide a desired oxygen concentration in the calibration fluid, and wherein the desired oxygen concentration is measured using the at least one electrode of the sensor array prior to contacting the at least pO 2  sensor with the calibration fluid for calibration thereof,   wherein the at least one calibration and/or quality control reagent comprising oxygen and the reagent comprising a pyrogallol oxygen scavenger are in aqueous solution,   wherein the at least one calibration and/or quality control reagent comprising oxygen and the reagent comprising a pyrogallol oxygen scavenger are disposed in a substantially air tight environment until use thereof.   
     
     
         12 . The kit of  claim 11 , wherein the pyrogallol comprises a concentration from about 0.05% to about 1% of the calibration fluid. 
     
     
         13 . The kit of  claim 11 , wherein the specific amount of the reagent comprising a pyrogallol oxygen scavenger is from about 0.1 milliliter to about 2 milliliters. 
     
     
         14 . The kit of  claim 11 , wherein the electrode is a bar metal electrode. 
     
     
         15 . The kit of  claim 11 , wherein the desired oxygen concentration comprises a partial pressure of oxygen of about zero millimeters of mercury (mmHg).