Biocidal Compositions Comprising Iron Chelators

Abstract

The present invention, therefore, is directed to an antimicrobial compositions that decreases the bioavailability of iron by introducing a higher-affinity iron-selective chelating agent capable of competing with microbial siderophores. In one aspect, the present invention relates to an antimicrobial composition including a potentiating antimicrobial composition including one or more antimicrobial agents and a chelator having a weight ratio of the antimicrobial agent to the chelator from about 1:1000 to about 1000:1.

What is claimed is: 
     
         1 . An antimicrobial composition comprising:
 a) at least one antimicrobial agent; and   b) an iron-chelator,   wherein the at least one antimicrobial agent and the iron chelator are present in a weight ratio of from about 1:1000 to about 1000:1.   
     
     
         2 . The antimicrobial composition of  claim 1 , wherein the at least one antimicrobial agent is selected from a group consisting of benzisothiazolinone, butylbenzisothiazolinone, iodopropynyl butylcarbamate, propiconazole, salicylic acid, and benzoyl peroxide. 
     
     
         3 . The antimicrobial composition of  claim 1 , wherein the at least one antimicrobial agent pyrithione salt is selected from a group consisting of sodium pyrithione, zinc pyrithione, chitosan pyrithione, magnesium disulfide pyrithione, and copper pyrithione. 
     
     
         4 . The antimicrobial composition of  claim 1 , wherein the iron-chelator is selected from a group consisting of alpha-amino carboxylates, hydroxamates, catechols, pyridinones, hydroxyquinolines, and salts thereof. 
     
     
         5 . The antimicrobial composition of  claim 4 , wherein the iron chelator is selected from a group consisting of ethylenediaminetetraacetic acid; diethylenetriaminepentaacetic acid; hydroxylpropylenediaminetetraacetic acid; N,N′-bis(o-hydroxybenzyl) ethylenediamine-N,N′diacteic acid; ethylenebis-N,N′-(2-o-hydroxyphenyl)glycine, 1,3-diaminopropane-N,N,N′,N′-tetraacetic acid; ethylenediamine-N,N′-diacetic acid; ethylenediamine-N,N′-dipropionic acid dihydrochloride; ethylenediamine-N,N′-bis(methylenephosphonic acid); N-(2-hydroxyethyl)ethylenediamine-N,N′,N′-triacetic acid; ethylenediamine-N,N,N′,N′-tetrakis(methylenephosponic acid); O,O′-bis(2-aminoethyl)ethyleneglycol-N,N,N′,N′-tetraacetic acid; N,N-bis(2-hydroxybenzyl)ethylenediamine-N,N-diacetic acid; 1,6-hexamethylenediamine-N,N,N′,N′-tetraacetic acid; N-(2-hydroxyethyl)iminodiacetic acid; iminodiacetic acid; 1,2-diaminopropane-N,N,N′,N′-tetraacetic acid; nitrilotriacetic acid; nitrilotripropionic acid; nitrilotris(methylenephosphonic acid); and triethylenetetramine—N,N,N′,N″,N′″,N′″-hexaacetic acid. 
     
     
         6 . The antimicrobial composition of  claim 4 , wherein the iron-chelator is selected from a group consisting of a barium, calcium, cobalt, copper, dysprosium, europium, iron, indium, lanthanum, magnesium, manganese, nickel, samarium, sodium, strontium, and zinc chelate of ethylenediaminetetraacetic acid; diethylenetriaminepentaacetic acid; hydroxylpropylenediaminetetraacetic acid; N,N′-bis(o-hydroxybenzyl) ethylenediamine-N,N′diacteic acid; ethylenebis-N,N′-(2-o-hydroxyphenyl)glycine, 1,3-diaminopropane-N,N,N′,N′-tetraacetic acid; ethylenediamine-N,N′-diacetic acid; ethylenediamine-N,N′-dipropionic acid dihydrochloride; ethylenediamine-N,N′-bis(methylenephosphonic acid); N-(2-hydroxyethyl)ethylenediamine-N,N′,N′-triacetic acid; ethylenediamine-N,N,N′,N′-tetrakis(methylenephosponic acid); O,O′-bis(2-aminoethyl)ethyleneglycol-N,N,N′,N′-tetraacetic acid; N,N-bis(2-hydroxybenzyl)ethylenediamine-N,N-diacetic acid; 1,6-hexamethylenediamine-N,N,N′,N′-tetraacetic acid; N-(2-hydroxyethyl)iminodiacetic acid; iminodiacetic acid; 1,2-diaminopropane-N,N,N′,N′-tetraacetic acid; nitrilotriacetic acid; nitrilotripropionic acid; nitrilotris(methylenephosphonic acid); and triethylenetetramine—N,N,N′,N″,N′″,N′″-hexaacetic acid. 
     
     
         7 . The antimicrobial composition of  claim 4 , wherein the iron-chelator is selected from a group consisting of ethylenebis-N,N′-(2-o-hydroxyphenyl)glycine, 2-[[2-bis(carboxymethyl)amino]-5-methylphenoxy]methyl]-8-[bis(carboxymethyl)amino]-quinoline, 2-[[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-6-methoxy-8-[bis(carboxy-methyl)amino]quinoline, O,O′-bis(2-aminoethyl)ethyleneglycol-N,N,N′,N′-tetraacetic acid, 8-hydroxyquinoline, 2-hydroxypyridine-1-oxide, salicylaldehyde isonicotinoyl hydrazone, thenoyl trifluoro acetone, dihydroxyacetate, tropolone, hydroxyethylidine-1,1′-diphosphonic acid, dehydroacetic acid, and glucoheptanoic acid. 
     
     
         8 . The antimicrobial composition of  claim 4 , wherein the iron-chelator is selected from a group consisting of a sodium, potassium, calcium, magnesium, copper, and zinc salt of ethylenebis-N,N′-(2-o-hydroxyphenyl)glycine, 2-[[2-bis(carboxymethyl)amino]-5-methylphenoxy]methyl]-8-bis(carboxymethyl)amino]-quinoline, 2-[[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-6-methoxy-8-[bis(carboxy-methyl)amino]quinoline, O,O′-bis(2-aminoethyl)ethyleneglycol-N,N,N′,N′-tetraacetic acid, 8-hydroxyquinoline, 2-hydroxypyridine-1-oxide, salicylaldehyde isonicotinoyl hydrazone, thenoyl trifluoro acetonedihydroxyacetate, tropolone, hydroxyethylidine-1,1′-diphosphonic acid, dehydroacetic acid, and glucoheptanoic acid. 
     
     
         9 . The antimicrobial composition of  claim 4 , wherein the iron-chelator is selected from a group consisting of diethylenetriaminepentaacetic acid; N,N′-bis(o-hydroxybenzyl) ethylenediamine-N,N′diacteic acid; 8-hydroxyquinoline; and 2-hydroxypyridine-1-oxide or selected from a group consisting of a sodium, potassium, calcium, magnesium, copper, and zinc salt of diethylenetriaminepentaacetic acid; N,N′-bis(o-hydroxybenzyl) ethylenediamine-N,N′diacteic acid; 8-hydroxyquinoline; and 2-hydroxypyridine-1-oxide. 
     
     
         10 . The antimicrobial composition of  claim 4 , wherein the iron-chelator is selected from a group consisting of a zinc salt of diethylenetriaminepentaacetic acid; N,N′-bis(o-hydroxybenzyl) ethylenediamine-N,N′diacteic acid; 8-hydroxyquinoline; and 2-hydroxypyridine-1-oxide. 
     
     
         11 . The antimicrobial composition of  claim 1 , wherein the at least one antimicrobial agent and the iron chelator are present in a weight ratio of from about 1:10 to about 10:1. 
     
     
         12 . The antimicrobial composition of  claim 1 , wherein the at least one antimicrobial agent and the iron chelator are present in a weight ratio of from about 1:10 to about 1:1. 
     
     
         13 . The antimicrobial composition of  claim 1 , wherein the at least one antimicrobial agent is present in an amount of from about 1 ppm to about 10,000 ppm and wherein the iron chelator is present in an amount of from about 10 ppm to about 2000 ppm, based on the weight of water in the system being treated. 
     
     
         14 . A personal care composition comprising the antimicrobial composition of  claim 1 , the personal care composition selected from the group consisting of a cream, an ointment, a lotion, a shampoo, a conditioner, a sunscreen, a deodorant, a soap, a hand cleaner, a detergent, a scrub, a bactericidal wash, and an acne formulation. 
     
     
         15 . A method of inhibiting microbial growth on a surface which comprises applying to said surface an effective amount of an antimicrobial composition comprising:
 a) at least one antimicrobial agent selected from a group consisting of zinc pyrithione, benzisothiazolinone, butylbenzisothiazolinone, iodopropynyl butylcarbamate, propiconazole, salicylic acid, and benzoyl peroxide; and   b) an iron-chelator selected from a group consisting of alpha-amino carboxylates, hydroxamates, catechols, pyridinones, hydroxyquinolines, and salts thereof,   wherein the at least one antimicrobial agent is present in an amount of from about 1 ppm to about 10,000 ppm and wherein the chelator is present in an amount of from about 10 ppm to about 2000 ppm, based on the weight of water in the system being treated.   
     
     
         16 . The method of  claim 15 , wherein the iron-chelator is selected from a group consisting of diethylenetriaminepentaacetic acid; N,N′-bis(o-hydroxybenzyl) ethylenediamine-N,N′diacteic acid; 8-hydroxyquinoline; and 2-hydroxypyridine-1-oxide or selected from a group consisting of a sodium, potassium, calcium, magnesium, copper, and zinc salt of diethylenetriaminepentaacetic acid; N,N′-bis(o-hydroxybenzyl) ethylenediamine-N,N′diacteic acid; 8-hydroxyquinoline; and 2-hydroxypyridine-1-oxide. 
     
     
         17 . The method of  claim 16 , wherein the iron-chelator is selected from a group consisting of a zinc salt of diethylenetriaminepentaacetic acid; N,N′-bis(o-hydroxybenzyl) ethylenediamine-N,N′diacteic acid; 8-hydroxyquinoline; and 2-hydroxypyridine-1-oxide. 
     
     
         18 . The method of  claim 15 , wherein the at least one antimicrobial agent is present in an amount of from about 10 ppm to about 1000 ppm and wherein the chelator is present in an amount of from about 100 ppm to about 500 ppm, based on the weight of water in the system being treated. 
     
     
         19 . The method of  claim 15 , wherein the at least one antimicrobial agent is present in an amount of from about 50 ppm to about 1000 ppm and wherein the chelator is present in an amount of from about 100 ppm to about 500 ppm, based on the weight of water in the system being treated. 
     
     
         20 . The method of  claim 15 , wherein the at least one antimicrobial agent and the iron chelator are present in a weight ratio of from about 1:10 to about 1:1.