Subtypes of humanized antibody against interleukin-6 receptor

Abstract

An antibody subtype (1) which is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which one C-terminal of the heavy chain is Pro-NH2 (447), and an antibody subtype (2) which is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which both C-terminals of the heavy chain are Pro-NH2 (447), and a pharmaceutical composition comprising them.

1 . An antibody subtype (1) which is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which one C-terminal of the heavy chain is Pro-NH 2  (447). 
     
     
         2 . An antibody subtype (2) which is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which both C-terminals of the heavy chain are Pro-NH 2  (447). 
     
     
         3 . The antibody subtype according to  claim 1  or  2  wherein the heavy chain of the native humanized PM-1 antibody corresponding to the subtype according to  claim 1  or  2  has an amino acid sequence set forth in SEQ ID NO: 1. 
     
     
         4 . The antibody subtype according to  claim 3  wherein glutamine (Gln) at the heavy chain N-terminal is pyroglutamic acid (pGlu). 
     
     
         5 . The antibody subtype according to any one of  claims 1  to  4  wherein the light chain of the above humanized PM-1 antibody subtype has an amino acid sequence set forth in SEQ ID NO: 2. 
     
     
         6 . A pharmaceutical composition comprising either the subtype (1) or the subtype (2) described in  claim 1 , or both of the subtypes (1) and (2). 
     
     
         7 . A method for the treatment of an IL-6R related disease in an individual, comprising administering to the individual the pharmaceutical composition comprising an antibody subtype (1) which is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which one C-terminal of the heavy chain is Pro-NH 2  (447). 
     
     
         8 . A method of producing an antibody subtype 1 comprising culturing a host cell comprising at least one nucleic acid encoding for a heavy chain and at least one nucleic acid encoding for a light chain of an anti-IL-6R antibody, wherein said antibody subtype 1 is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which one C-terminal of the heavy chain is Pro-NH 2  (447).