Method of manufacturing recombinant lectin protein and recombinant lectin protein manufactured by using the same

Abstract

Provided is a recombinant BPL3 protein having β-GlcNAc-Sp and a glycan binding specificity, the recombinant BPL3 protein produced by synthesizing a recombinant BPL3 (rBPL3) gene encoding a Bryopsis plumosa lectin (BPL3) protein; preparing a tandem repeat rBPL3 gene including a repeating structure of the rBPL3 gene by tandemly binding the rBPL3 genes through spacers; preparing a recombinant plasmid by inserting the tandem repeat rBPL3 gene into an expression vector; and transforming an expression host by the recombinant plasmid. According to the method, the expression efficiency of the recombinant lectin is maximized and the activity of the manufactured recombinant lectin is enhanced.

What is claimed is: 
     
         1 . A recombinant BPL3 protein comprising β-GlcNAc-Sp and a glycan binding specificity,
 wherein the recombinant BPL3 protein is produced by:
 synthesizing a recombinant  Bryopsis plumosa  lectin (rBPL3) gene encoding a  Bryopsis plumosa  lectin (BPL3) protein; 
 preparing a tandem repeat rBPL3 gene including a repeating structure of the rBPL3 gene by tandemly binding the rBPL3 genes through spacers; 
 preparing a recombinant plasmid by inserting the tandem repeat rBPL3 gene into an expression vector; and 
 transforming an expression host with the recombinant plasmid.