US2020095542A1PendingUtilityA1

Culture medium composition

Assignee: NISSAN CHEMICAL CORPPriority: Jan 23, 2014Filed: Oct 24, 2019Published: Mar 26, 2020
Est. expiryJan 23, 2034(~7.5 yrs left)· nominal 20-yr term from priority
C12N 2533/72C12N 2535/00C12M 25/16C12N 2533/70C12N 2533/78C12N 5/0068C12N 2509/10A01N 1/122
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Claims

Abstract

The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Claims

exact text as granted — not AI-modified
1 . A liquid composition comprising:
 (1) a culture medium,   (2) an adherent cell, and   (3) a chitin nanofiber,   
       wherein the chitin nanofiber is dispersed in the culture medium and form a three-dimensional network in the culture medium so that the adherent cell can be dispersed while spreading three-dimensionally and proliferate in the state of being attached to the chitin nanofiber. 
     
     
         2 . The liquid composition of  claim 1 , wherein the liquid composition has a chitin nanofiber content of 0.001% (weight/volume) to 0.1% (weight/volume).

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