Therapeutically active compounds and their methods of use

Abstract

Provided are compounds useful for treating cancer and methods of treating cancer comprising administering to a subject in need thereof a compound described herein.

1 - 19 . (canceled) 
     
     
         20 . A methanesulfonate salt of a compound having formula I: 
       
         
           
           
               
               
           
         
       
       wherein:
 ring A is selected from phenyl, oxazolyl, isoxazolyl, pyridinyl, pyrimidinyl, and thiazolyl, wherein ring A is optionally substituted with up to two substituents independently selected from halo, C 1 -C 4  alkyl, C 1 -C 4  haloalkyl, C 1 -C 4  hydroxyalkyl, —NH—S(O) 2 —C 1 -C 4  alkyl, —S(O) 2 —NH(C 1 -C 4  alkyl), CN, S(O) 2 —C 1 -C 4  alkyl, C 1 -C 4  alkoxy, NH(C 1 -C 4  alkyl), —OH, and —NH 2 ; 
 ring B is an optionally substituted 5-6 member monocyclic aryl or monocyclic heteroaryl; 
 R 1  and R 3  are each independently selected from hydrogen, C 1 -C 4  alkyl, C 1 -C 4  haloalkyl, —O—C 1 -C 4  alkyl, and CN, wherein any alkyl portion of R 1  is optionally substituted with —OH, NH 2 , NH(C 1 -C 4  alkyl), or N(C 1 -C 4  alkyl) 2 ; 
 R 2  is selected from: —(C 1 -C 6  alkyl), —(C 2 -C 6  alkenyl or alkynyl), —(C 1 -C 6  alkylene)-N(R 6 )—(C 1 -C 6  alkylene)-O—(C 1 -C 6  alkyl), —(C 1 -C 6  alkylene)-N(R 6 )—(C 0 -C 6  alkylene)-Q, —(C 1 -C 6  alkylene)-N(R 6 )(R 6 ), —(C 1 -C 6  alkylene)-N(R 6 )—S(O) 1-2 —(C 1 -C 6  alkyl), —(C 1 -C 6  alkylene)-N(R 6 )—S(O) 1-2 —(C 0 -C 6  alkyl)-Q, —(C 1 -C 6  alkylene)-S(O) 1-2 —N(R 6 )(R 6 ), —(C 1 -C 4  alkylene)-S(O) 1-2 —N(R 6 )—(C 1 -C 6  alkylene)-Q, —C(O)N(R 6 )—(C 1 -C 6  alkylene)-C(O)— (C 0 -C 6  alkylene)-O—(C 1 -C 6  alkyl), —C(O)N(R 6 )—(C 1 -C 6  alkylene)-C(O)—(C 0 -C 6  alkylene)-O—(C 0 -C 6  alkylene)-Q, —(C 1 -C 6  alkylene)-O—C(O)—(C 1 -C 6  alkyl), —(C 1 -C 6  alkylene)-O—C(O)—(C 0 -C 6  alkyl)-Q, —(C 1 -C 6  alkylene)-O—(C 1 -C 6  alkyl), —(C 1 -C 6  alkylene)-O—(C 1 -C 6  alkylene)-Q, —(C 0 -C 6  alkylene)-C(O)—(C 0 -C 6  alkylene)-O—(C 1 -C 6  alkyl), —(C 0 -C 6  alkylene)-C(O)—(C 0 -C 6  alkylene)-O—(C 1 -C 6  alkylene)-Q, —(C 1 -C 6  alkylene)-O—C(O)—(C 1 -C 6  alkyl), —(C 1 -C 6  alkylene)-O—C(O)—(C 0 -C 6  alkylene)-Q, —(C 0 -C 6  alkylene)-C(O)N(R 6 )—(C 1 -C 6  alkyl), —(C 0 -C 6  alkylene)-C(O)N(R 6 )—(C 0 -C 6  alkylene)-Q, —(C 1 -C 6  alkylene)-N(R 6 )C(O)—(C 1 -C 6  alkyl), —(C 1 -C 6  alkylene)-N(R 6 )C(O)—(C 0 -C 6  alkylene)-Q, —(C 0 -C 6  alkylene)-S(O) 0-2 —(C 1 -C 6  alkyl), —(C 0 -C 6  alkylene)-S(O) 0-2 —(C 0 -C 6  alkylene)-Q, —(C 1 -C 6  alkylene)-N(R 6 )—C(O)—N(R 6 )—(C 1 -C 6  alkyl), —(C 0 -C 6  alkylene)-Q, —(C 0 -C 6  alkylene)-C(O)—(C 1 -C 6  alkyl), —(C 0 -C 6  alkylene)-C(O)—(C 0 -C 6  alkylene)-Q, wherein: 
 any alkyl or alkylene moiety present in R 2  is optionally substituted with one or more —OH, —O(C 1 -C 4  alkyl) or halo; 
 any terminal methyl moiety present in R 2  is optionally replaced with —CH 2 OH, CF 3 , —CH 2 F, —CH 2 Cl, C(O)CH 3 , C(O)CF 3 , CN, or CO 2 H; 
 each R 6  is independently selected from hydrogen and C 1 -C 6  alkyl; and 
 Q is selected from aryl, heteroaryl, carbocyclyl and heterocyclyl, any of which is optionally substituted; or 
 R 1  and R 3  are optionally taken together with the carbon to which they are attached to form C(═O), or 
 R 1  and R 2  are optionally taken together to form substituted carbocyclyl, or optionally substituted heterocyclyl, wherein: 
 (a) when ring A is unsubstituted phenyl, and ring B is phenyl substituted by methoxy or ethoxy; then said phenyl of ring B is not further substituted by oxazolyl; 
 (b) when ring A is optionally substituted phenyl or optionally substituted pyridyl and ring B is optionally substituted phenyl; then the portion of the compound represented by —NH—C(R 1 )(R 2 )(R 3 ) is not —NH(CH 2 )-aryl; 
 (c) when ring A is optionally substituted phenyl, and ring B is optionally substituted phenyl or pyrrolyl; then the portion of the compound represented by —NH—C(R 1 )(R 2 )(R 3 ) is not —NH(CH 2 )C(O)NH 2 ; 
 (d) when ring A is phenyl substituted with 2 hydroxyl or methoxy, and ring B is optionally substituted phenyl; then the portion of the compound represented by —NH—C(R 1 )(R 2 )(R 3 ) is not —NH-cycloheptyl; 
 (e) when ring A is optionally substituted phenyl and ring B is optionally substituted phenyl; then R 1  and R 2  do not form 2,2,6,6-tetramethylpiperidin-4-yl; 
 (f) when ring A and ring B are optionally substituted phenyl; then the portion of the compound represented by —NH—C(R 1 )(R 2 )(R 3 ) is not cysteine, optionally substituted phenylalanine or leucine; or methyl ester thereof; 
 (g) when ring A is phenyl or pyridin-3-yl optionally substituted with one or more substituents selected from halo, methyl or CF 3 , and ring B is phenyl optionally substituted with one or more substituents selected from halo, methyl or CF 3 , methoxy, or CH═C(phenyl)CN; then the portion of the compound represented by —NHC(R 1 )(R 2 )(R 3 ) is other than —NH(C 1 -C 8  alkylene)-N(R a )(R a ), —NH-1-(aminomethyl)cyclopentylmethyl, —NH-4-(aminomethyl)cyclohexylmethyl, wherein each R a  is hydrogen, C 1 -C 4  alkyl or two R a s are taken together with the nitrogen to which they are commonly bound to form morpholin-4-yl or piperidin-1-yl; 
 (h) when ring A is phenyl, 4-chlorophenyl or 4-methylphenyl and ring B is 4-chlorophenyl or 3,4-dichlorophenyl; then the portion of the compound represented by —NHC(R 1 )(R 2 )(R 3 ) is not —NH-isopropyl; 
 (i) when ring A is unsubstituted phenyl and the portion of the compound represented by —NHC(R 1 )(R 2 )(R 3 ) is —NH—CH 2 CH 2 N(CH 3 ) 2 , —NH—CH 2 CH 2 -morpholin-4-yl or —NH—CH 2 CH 2 OH; then ring B is other than oxadiazole, imidazole, thiazole or oxazole each of which is substituted with —C(O)NHR b , wherein R b  is isopropyl, cyclopropyl or 2-chloro-6-methylphenyl; and 
 (j) the compound is other than: 
 (E)-3-(4-((4-((3-(diethylamino)propyl)amino)-6-phenyl-1,3,5-triazin-2-yl)amino)-2-methoxyphenyl)-2-phenylacrylonitrile, 
 4-((4-((furan-2-ylmethyl)amino)-6-(pyridin-4-yl)-1,3,5-triazin-2-yl)amino)phenol, 
 3-(4-((5-aminopentyl)amino)-6-((3-fluorophenyl)amino)-1,3,5-triazin-2-yl)phenol, 
 N 2 ,6-bis(3-fluorophenyl)-N 4 -(piperidin-3-yl)-1,3,5-triazine-2,4-diamine, 
 N 2 -butyl-6-phenyl-N 4 -(p-tolyl)-1,3,5-triazine-2,4-diamine, 
 N 2 -cyclohexyl-N 4 ,6-diphenyl-1,3,5-triazine-2,4-diamine, 
 (R)-3-((4-(3-chlorophenyl)-6-(pyrrolidin-3-ylamino)-1,3,5-triazin-2-yl)amino)-4-methylbenzamide, 
 2-chloro-4-(methylsulfonyl)-N-[4-(phenylamino)-6-(2-pyridinyl)-1,3,5-triazin-2-yl]-benzamide, 
 N 2 -(2-methoxyethyl)-N 4 -phenyl-6-[5-[6-(2,2,2-trifluoroethoxy)-3-pyridinyl]-1,2,4-oxadiazol-3-yl]1,3,5-triazine-2,4-diamine, 
 N 2 -(2-furanylmethyl)-6-phenyl-N 4 -[3-(trifluoromethyl)phenyl]-1,3,5-triazine-2,4-diamine, 
 6-(3-methoxyphenyl)-N 2 -methyl-N 4 -(3-nitrophenyl)-1,3,5-triazine-2,4-diamine, N 2 -butyl-N 4 -(4-methylphenyl)-6-phenyl-1,3,5-triazine-2,4-diamine, and 
 4-[4-(5-chloro-2-methylphenyl)-6-(methylamino)-1,3,5-triazin-2-yl]amino-benzenemethanol. 
 
     
     
         21 . The methanesulfonate salt of  claim 20 , wherein R 1  is independently selected from hydrogen, —CH 3 , —CH 2 CH 3 , CH 2 OH, CN, or R 1  and R 3  are taken together to form ═O. 
     
     
         22 . The methanesulfonate salt of  claim 20 , wherein R 1  and R 2  are taken together to form substituted carbocyclyl or optionally substituted heterocyclyl, wherein the substituents, when present, are independently selected from halo, C 1 -C 4  alkyl, C 1 -C 4  haloalkyl, C 1 -C 4  alkoxy, CN, ═O, —OH, and —C(O)C 1 -C 4  alkyl. 
     
     
         23 . The methanesulfonate salt of  claim 20 , wherein R 2  is selected from: —(C 1 -C 4  alkyl) optionally substituted with fluoro or —OH; —(C 0 -C 4  alkylene)-O—(C 1 -C 4  alkyl), —(C 0 -C 2  alkylene)-N(R 6 )—(C 1 -C 6  alkyl), —(C 0 -C 2  alkylene)-Q, and —O—(C 0 -C 2  alkylene)-Q, wherein Q is optionally substituted with up to 3 substituents independently selected from C 1 -C 4  alkyl, C 1 -C 4  haloalkyl, C 1 -C 4  alkoxy, ═O, —C(O)—C 1 -C 4  alkyl, —CN, and halo. 
     
     
         24 . The methanesulfonate salt of  claim 20 , wherein ring B is selected from phenyl, pyrazolyl, oxazolyl, isoxazolyl, pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, and thiazolyl, wherein ring B is optionally substituted with up to two substituents independently selected from halo, C 1 -C 4  alkyl, C 2 -C 4  alkynyl, C 1 -C 4  haloalkyl, C 1 -C 4  hydroxyalkyl, C 3 -C 6  cycloalkyl, (C 0 -C 2  alkylene)-O—C 1 -C 4  alky), —O—(C 1 -C 4  alkylene)-C 3 -C 6  cycloalkyl, —NH—S(O) 2 —(C 1 -C 4  alkyl), —S(O) 2 —NH(C 1 -C 4  alkyl), —S(O) 2 —NH—(C 3 -C 6  cycloalkyl), —S(O) 2 -(saturated heterocyclyl), —CN, —S(O) 2 —(C 1 -C 4  alkyl), —NH(C 1 -C 4  alkyl), —NH(C 1 -C 4  alkyl) 2 , —OH, C(O)—O—(C 1 -C 4  alkyl), saturated heterocyclyl, and —NH 2 . 
     
     
         25 . The methanesulfonate salt of  claim 20 , wherein
 R 1  is independently selected from hydrogen, —CH 3 , —CH 2 CH 3 , CH 2 OH, CN, or R 1  and R 3  are taken together to form ═O;   R 2  is selected from: —(C 1 -C 4  alkyl) optionally substituted with fluoro or —OH; —(C 0 -C 4  alkylene)-O—(C 1 -C 4  alkyl), —(C 0 -C 2  alkylene)-N(R 6 )—(C 1 -C 6  alkyl), —(C 0 -C 2  alkylene)-Q, and —O—(C 0 -C 2  alkylene)-Q, wherein Q is optionally substituted with up to 3 substituents independently selected from C 1 -C 4  alkyl, C 1 -C 4  haloalkyl, C 1 -C 4  alkoxy, ═O, —C(O)—C 1 -C 4  alkyl, —CN, and halo; and   B is selected from phenyl, pyrazolyl, oxazolyl, isoxazolyl, pyridinyl, pyrimidinyl, pyridazinyl, pyrazinyl, and thiazolyl, wherein ring B is optionally substituted with up to two substituents independently selected from halo, C 1 -C 4  alkyl, C 2 -C 4  alkynyl, C 1 -C 4  haloalkyl, C 1 -C 4  hydroxyalkyl, C 3 -C 6  cycloalkyl, (C 0 -C 2  alkylene)-O—C 1 -C 4  alky), —O—(C 1 -C 4  alkylene)-C 3 -C 6  cycloalkyl, —NH—S(O) 2 —(C 1 -C 4  alkyl), —S(O) 2 —NH(C 1 -C 4  alkyl), —S(O) 2 —NH—(C 3 -C 6  cycloalkyl), —S(O) 2 -(saturated heterocyclyl), —CN, —S(O) 2 —(C 1 -C 4  alkyl), —NH(C 1 -C 4  alkyl), —NH(C 1 -C 4  alkyl) 2 , —OH, C(O)—O—(C 1 -C 4  alkyl), saturated heterocyclyl, and —NH 2 .   
     
     
         26 . The methanesulfonate salt of  claim 20 , wherein the compound of formula I is selected from 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
       
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
       
     
     
         27 . A pharmaceutical composition comprising the methanesulfonate salt of  claim 20 , and a pharmaceutically acceptable carrier. 
     
     
         28 . A method of treating a cancer characterized by the presence of an IDH2 mutation, wherein the IDH2 mutation results in a new ability of the enzyme to catalyze the NAPH dependent reduction of α ketoglutarate to R(−) 2 hydroxyglutarate in a patient, comprising the step of administering to the patient in need thereof the methanesulfonate salt of  claim 20 . 
     
     
         29 . The method of  claim 28 , wherein the IDH2 mutation is an IDH2 R140Q or R172K mutation. 
     
     
         30 . The method of  claim 28 , wherein the IDH2 mutation is an IDH2 R140Q mutation. 
     
     
         31 . The method of  claim 28 , wherein the cancer is selected from glioblastoma, myelodysplastic syndrome, myeloproliferative neoplasm, acute myelogenous leukemia, sarcoma, melanoma, non-small cell lung cancer, chondrosarcoma, cholangiocarcinomas or angioimmunoblastic non-Hodgkin's lymphoma. 
     
     
         32 . The method of  claim 28 , wherein the cancer is acute myelogenous leukemia. 
     
     
         33 . The method of  claim 28 , further comprising administering to the patient in need thereof a second therapeutic agent useful in the treatment of cancer.