US2020056158A1PendingUtilityA1

Method for screening for pluripotent stem cell growth-promoting factor

Assignee: KANEKA CORPPriority: Jun 28, 2013Filed: Jul 23, 2019Published: Feb 20, 2020

Est. expiryJun 28, 2033(~6.9 yrs left)· nominal 20-yr term from priority

Inventors:Tomohisa Kato Yonehiro Kanemura Tomoko Shofuda Hayato Fukusumi

C12N 2500/25C12N 2500/99G01N 33/5073C12N 2501/115C12N 5/0696C12N 2501/15

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Claims

Abstract

Disclosed are a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.

Claims

exact text as granted — not AI-modified

1 . A method for growing pluripotent stem cells comprising:
 a) culturing pluripotent stem cells on feeder cells using a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain serum replacement;   b) recovering the pluripotent stem cells cultured on the feeder cells; and   c) culturing the pluripotent stem cells in a feeder-free medium.   
     
     
         2 . The method according to  claim 1 , wherein the serum-free medium does not contain albumin.

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