Composition for culturing brain organoid based on decellularized brain matrix and method for preparing same

Abstract

Provided is a method for preparing a composition for culturing a brain organoid, the method comprising (a) decellularizing brain tissue; (b) drying the brain tissue; and (c) gelating the brain tissue.

What is claimed is: 
     
         1 . A method for preparing a composition for culturing a brain organoid, comprising:
 (a) decellularizing brain tissue;   (b) drying the brain tissue; and   (c) gelating the brain tissue.   
     
     
         2 . The method according to  claim 1 ,
 wherein in the (c), the brain tissue and Matrigel are mixed to obtain a hydrogel.   
     
     
         3 . The method according to  claim 2 ,
 wherein the hydrogel contains a decellularized brain extracellular matrix (dBEM) in an amount of 0.01 to 2.0 mg/mL.   
     
     
         4 . The method according to  claim 1 ,
 wherein in the (a), the brain tissue is stirred in a decellularizing solution.   
     
     
         5 . The method according to  claim 4 ,
 wherein the brain tissue is stirred for 3 to 24 hours.   
     
     
         6 . The method according to  claim 4 ,
 wherein 95% or more of cells of the brain tissue is removed by the decellularization.   
     
     
         7 . A hydrogel composition for culturing a brain organoid, comprising:
 a decellularized brain extracellular matrix (dBEM).   
     
     
         8 . The hydrogel composition according to  claim 7 , further comprising:
 Matrigel.   
     
     
         9 . The hydrogel composition according to  claim 7 ,
 wherein the decellularized brain extracellular matrix (dBEM) has a concentration of 0.01 to 2.0 mg/mL.   
     
     
         10 . The hydrogel composition according to  claim 7 , which has an elastic modulus at 1 Hz of 100 to 150 Pa. 
     
     
         11 . A method for culturing a brain organoid in the composition of  claims 7 .