US2019375749A1PendingUtilityA1

Kras g12c inhibitors and methods of using the same

Assignee: AMGEN INCPriority: Jun 11, 2018Filed: Jun 10, 2019Published: Dec 12, 2019
Est. expiryJun 11, 2038(~11.9 yrs left)· nominal 20-yr term from priority
A61K 38/16A61P 35/00C07D 471/04A61K 45/06
63
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Claims

Abstract

composition of the same, and methods of using the same. These inhibitors are useful for treating a number of disorders, including pancreatic, colorectal, and lung cancers.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A compound having a structure of formula (I) 
       
         
           
           
               
               
           
         
         wherein 
         E 1  and E 2  are each independently N or CR 1 ;
    is a single or double bond as necessary to give every atom its normal valence; 
 R 1  is independently H, hydroxy, —C 1-6 alkyl, —C 1-6 haloalkyl, —C 1-6 alkoxy, —NH—C 1-6 alkyl, —N(C 1-4 alkyl) 2 , cyano, or halo; 
 R 2  is halo, —C 1-6 alkyl, —C 1-6 haloalkyl, —OR 2a , —N(R 2a ) 2 , —C 2-6 alkenyl, —C 2-6 alkynyl, —C 0-3 alkylene-C 3-14 cycloalkyl, —C 0-3 alkylene-C 2-14 heterocycloalkyl, aryl, heteroaryl, —C 0-3 alkylene-C 6-14 aryl, or —C 0-3 alkylene-C 2-14 heteroaryl, and each R 2a  is independently H, —C 1-6 alkyl, —C 1-6 haloalkyl, —C 3-14 cycloalkyl, —C 2-14 heterocycloalkyl, —C 2-6 alkenyl, —C 2-6 alkynyl, aryl, or heteroaryl, or two R 2a  substituents, together with the nitrogen atom to which they are attached, form a 3-7-membered ring; 
 R 3  is halo, —C 1-6 alkyl, —C 1-6 haloalkyl, —C 1-6 alkoxy, C 3-6 cycloalkyl, —C 2-14 heterocycloalkyl, —C 2-6 alkenyl, —C 2-6 alkynyl, aryl, or heteroaryl; 
 R 4  is 
 
       
       
         
           
           
               
               
           
         
         
           ring A is a monocyclic 4-7 membered ring or a bicyclic, bridged, fused, or spiro 6-11 membered ring; 
           L is a bond, —C 1-6 alkylene, —O—C 0-6 alkylene, —S—C 0-6 alkylene, or —NH—C 0-6 alkylene, and for —C 2-6 alkylene, —O—C 2-6 alkylene, —S—C 2-6 alkylene, and NH—C 2-6  alkylene, one carbon atom of the alkylene group can optionally be replaced with O, S, or NH; 
           R 4a  is H, C 1-6 alkyl, C 2-6 alkynyl, C 1-6 alkylene-O—C 1-4 alkyl, C 1-6 alkylene-OH, C 1-6  haloalkyl, cycloalkyl, heterocycloalkyl, C 0-3 alkylene-C 3-14 cycloalkyl, C 0-3 alkylene-C 2-14  heterocycloalkyl, aryl, heteroaryl, C 0-3 alkylene-C 6-14 aryl, or selected from 
         
       
       
         
           
           
               
               
           
         
         
           R 5  and R 6  are each independently H, halo, —C 1-6 alkyl, —C 2-6 alkynyl, —C 1-6  alkylene-O—C 1-4 alkyl, —C 1-6 alkylene-OH, —C 1-6 haloalkyl, —C 1-6 alkyleneamine, —C 0-6  alkylene-amide, —C 1-3 alkylene-C(O)OH, —C 0-3 alkylene-C(O)OC 1-4 alkyl, —C 1-6  alkylene-O-aryl, —C 0-3 alkylene-C(O)C 1-4 alkylene-OH, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, —C 0-3 alkylene-C 3-14 cycloalkyl, —C 0-3 alkylene-C 2-14 heterocycloalkyl, —C 0-3 alkylene-C 6-14 aryl, —C 0-3 alkylene-C 2-14 heteroaryl, or cyano, or R 5  and R 6 , together with the atoms to which they are attached, form a 4-6 membered ring; 
           R 7  is H or C 1-6 alkyl, or R 7  and R 5 , together with the atoms to which they are attached, form a 4-6 membered ring; 
           R 8  is H, —C 1-6 alkyl, —C 0-3 alkylene-C 6-14 aryl, —C 0-3 alkylene-C 3-14 heteroaryl, —C 0-3  alkylene-C 3-14  cycloalkyl, —C 0-3 alkylene-C 2-14 heterocycloalkyl, —C 1-6 alkoxy, —O—C 0-3  alkylene-C 6-14 aryl, —O—C 0-3 alkylene-C 3-14 heteroaryl, —O—C 0-3  alkylene-C 3-14 cycloalkyl, —O—C 0-3 alkylene-C 2-14 heterocycloalkyl, —NH—C 1-8 alkyl, —N(C 1-8 alkyl) 2 , —NH—C 0-3 alkylene-C 6-14 aryl, —NH—C 0-3 alkylene-C 2-14 heteroaryl, —NH—C 0-3 alkylene-C 3-14 cycloalkyl, —NH—C 0-3  alkylene-C 2-14 heterocycloalkyl, halo, cyano, or C 1-6 alkylene-amine; 
           wherein the heteroaryl, spiroheterocycloalkyl and heterocycloalkyl groups of any of the R 2 , R 2a , R 3 , R 4 , R 4a , R 5 , R 6 , R 7 , and R 8  substituents have 1, 2, 3 or 4 heteroatoms independently selected from O, N or S, wherein the cycloalkyl, spirocycloalkyl, spiroheterocycloalkyl, and heterocycloalkyl groups may include a C═O group, and further wherein the spiroheterocycloalkyl, and heterocycloalkyl groups may include a S═O or SO 2 ; 
           wherein the —C 1-6 alkyl, —C 2-6 alkenyl, —C 2-6 alkynyl and the —OC 1-6 alkyl of any of the R 1 , R 2 , R 2a , R 3 , R 4 , R 4a , L, R 5 , R 6 , R 7 , and R 8  substituents is unsubstituted or substituted by 1, 2 or 3 R 9  substituents independently selected from OH, —OC 1-6 alkyl, —C 1-6 alkyl-O—C 1-6 alkyl, halo, —O-haloC 1-6 alkyl, —CN, —NR a R b , —(NR a R b R c ) n , —OSO 2 R a , —SO 2 R a , —(CH 2 CH 2 O) n CH 3 , -(═O), —C(═O), 
            —C(═O)R a , —OC(═O)R a , —C(═O)OR a , —C(═O)NR a R b , —O—SiR a R b R c , —SiR a R b R c , —O-(3- to 10-membered heterocycloakyl), a 6- to 12-membered aryl or heteroaryl, a 5- to 12-membered spirocycloalkyl or spiroheterocycloalkyl, a 3- to 12-membered cycloalkenyl, a 3- to 12-membered monocyclic or bicyclic cycloalkyl, or a 3- to 12-membered monocyclic or bicyclic heterocycloalkyl group, wherein the heteroaryl, spiroheterocycloalkyl and heterocycloalkyl groups have 1, 2, 3 or 4 heteroatoms independently selected from O, N or S, wherein the cycloalkyl, spirocycloalkyl, spiroheterocycloalkyl, and heterocycloalkyl groups may include a C═O group, and further wherein the spiroheterocycloalkyl, and heterocycloalkyl groups may include a S═O or SO 2 ; 
           wherein the aryl, heteroaryl, cycloalkyl, and heterocycloalkyl group of any of the R 1 , R 2 , R 2a , R 3 , R 4 , R 4a , R 5 , R 6 , R 7 , R 8  and R 9  substituents can be unsubstituted or substituted with 1, 2, 3 or 4 R 10  substituents independently selected from OH, halo, —NR c R d , —C 1-6 alkyl, —OC 1-6 alkyl, —C 1-6 alkyl-OH, —C 1-6 alkyl-O—C 1-6 alkyl, C 1-6 haloalkyl, —O-haloC 1-6 alkyl, —SO 2 R c , —CN, —C(═O)NR c R d , —C(═O)R c , —OC(═O)R a , —C(═O)OR c , a 6- to 12-membered aryl or heteroaryl, a 5- to 12-membered spirocycloalkyl or spiroheterocycloalkyl, a 3- to 12-membered cycloalkenyl, a 3- to 12-membered monocyclic or bicyclic cycloalkyl, or a 3- to 12-membered monocyclic or bicyclic heterocycloalkyl group, wherein the heteroaryl, spiroheterocycloalkyl, and heterocycloalkyl groups of R 10  have 1, 2, 3 or 4 heteroatoms independently selected from O, N or S, wherein the cycloalkyl, spirocycloalkyl, and spiroheterocycloalkyl groups of R 10  or the heterocycloalkyl group of R 10  may include a C═O group, and further wherein the spiroheterocycloalkyl and heterocycloalkyl groups may include a S═O or SO 2 ; 
           wherein each R a , R b , R c  and R d  is independently hydrogen, OH, —C 1-6 alkyl, —(CH 2 CH 2 O) n CH 3 , —NR 11 R 11 , —C 1-6 alkyl-NR 11 R 11 , phenyl, —C 1-6 alkyl-C(═O)OH, —C 1-6 alkyl-C(═O)—O—C 1-6 alkyl, —C 1-6 alkyl-3- to 12-membered cycloalkyl, —C 1-6 alkyl-3- to 12-membered heterocycloalkyl, —C 1-6 alkyl-6- to 12-membered heteroaryl, a 6- to 12-membered aryl or heteroaryl, a 3- to 12-membered monocyclic or bicyclic cycloalkyl, or a 3- to 12-membered monocyclic or bicyclic heterocycloalkyl group, wherein the heteroaryl group, heterocycloalkyl group of R a , R b , R c , and R d  or the heterocycloalkyl group of the —C 1-6 alkyl-heterocycloalkyl group of R a , R b , R c , and R d  has from 1, 2, 3, or 4 heteroatoms independently selected from O, N or S, wherein the cycloalkyl and heterocycloalkyl groups of R a , R b , R c , and R d  and the heterocycloalkyl group of the —C 1-6 alkyl-heterocycloalkyl groups of R a , R b , R c , and R d  may include a double bond, and further wherein the cycloalkyl and heterocycloalkyl groups of R a , R b , R c , and R d  and the heterocycloalkyl group of the —C 1-6 alkyl-heterocycloalkyl groups of R a , R b , R c , and R d  may contain a C═O group; 
           the alkyl, aryl, heteroaryl, cycloalkyl, heterocycloalkyl groups of R a , R b , R c , and R d  or the heterocycloalkyl groups of the —C 1-6 alkyl-heterocycloalkyl groups of R a , R b , R c , and R d  can be unsubstituted or substituted with from 1, 2, 3, or 4 R 12  substituents, wherein each R 12  is independently selected from H, OH, halo, —C 1-6 alkyl, N(CH 3 ) 2 , —C 1-6 haloalkyl, C(═O)CH 3 , —C(═O)OCH 3 , or —C 1-6 alkyl-O—C 1-6 alkyl; or 
           a stereoisomer thereof, an atropisomer thereof, a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable salt of the stereoisomer thereof, or a pharmaceutically acceptable salt of the atropisomer thereof. 
         
       
     
     
         2 . A compound of  claim 1  having a structure of formula (Ia) 
       
         
           
           
               
               
           
         
         a stereoisomer thereof, an atropisomer thereof, a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable salt of the stereoisomer thereof, or a pharmaceutically acceptable salt of the atropisomer thereof. 
       
     
     
         3 . The compound of  claim 1  wherein E 1  is N. 
     
     
         4 . The compound of  claim 1  wherein E 2  is CR 1 . 
     
     
         5 . The compound of  claim 4  wherein R 1  is H. 
     
     
         6 . The compound of  claim 1  wherein R 2  is selected from a halo, or an unsubstituted or substituted aryl. 
     
     
         7 . The compound of  claim 6  wherein R 2  is a substituted aryl. 
     
     
         8 . The compound of  claim 6  wherein R 2  is a fluorinated phenyl. 
     
     
         9 . The compound of  claim 6  wherein R 2  is Cl. 
     
     
         10 . The compound of  claim 6  wherein R 2  is 
       
         
           
           
               
               
           
         
       
     
     
         11 . The compound of  claim 6  wherein R 2  is 
       
         
           
           
               
               
           
         
       
     
     
         12 . The compound of  claim 1  wherein R 3  is halo. 
     
     
         13 . The compound of  claim 12  wherein R 3  is Cl. 
     
     
         14 . The compound of  claim 12  wherein R 3  is F. 
     
     
         15 . The compound of  claim 1  wherein R 4  is 
       
         
           
           
               
               
           
         
       
     
     
         16 . The compound of  claim 15  wherein L is a bond. 
     
     
         17 . The compound of  claim 15  wherein ring A is a monocyclic 4-7 membered ring. 
     
     
         18 . The compound of  claim 17  wherein A is an unsubstituted or substituted heterocycle. 
     
     
         19 . The compound of  claim 1 , wherein R 4  is selected from the group consisting of 
       
         
           
           
               
               
           
         
       
     
     
         20 . The compound of  claim 19 , wherein R 4  is 
       
         
           
           
               
               
           
         
       
     
     
         21 . The compound of  claim 19 , wherein R 4  is 
       
         
           
           
               
               
           
         
       
     
     
         22 . The compound of  claim 19 , wherein R 4  is 
       
         
           
           
               
               
           
         
       
     
     
         23 . The compound of  claim 1  wherein R 8  is —C 0-3 alkylene-C 6-14 aryl, or —C 0-3 alkylene-C 3-14 heteroaryl. 
     
     
         24 . The compound of  claim 23  wherein R 8  is —C 3-14 heteroaryl. 
     
     
         25 . The compound of  claim 23 , wherein R 8  is selected from the group consisting of 
       
         
           
           
               
               
           
         
       
     
     
         26 . A compound having a structure selected from the formula: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
       
       or a stereoisomer thereof, an atropisomer thereof, a pharmaceutically acceptable salt thereof, a pharmaceutically acceptable salt of the stereoisomer thereof, or a pharmaceutically acceptable salt of the atropisomer thereof. 
     
     
         27 . The compound of  claim 1  in the form of a pharmaceutically acceptable salt. 
     
     
         28 . A pharmaceutical composition comprising the compound of  claim 1  and a pharmaceutically acceptable excipient. 
     
     
         29 . A method of inhibiting KRAS G12C in a cell, comprising contacting the cell with the compound of  claim 1 . 
     
     
         30 . A method of treating cancer in a subject comprising administering to the subject a therapeutically effective amount of the compound of  claim 1 . 
     
     
         31 . The method of  claim 30 , wherein the cancer is lung cancer, pancreatic cancer, or colorectal cancer. 
     
     
         32 . The method of  claim 31 , wherein the cancer is lung cancer. 
     
     
         33 . The method of  claim 31 , wherein the cancer is pancreatic cancer. 
     
     
         34 . The method of  claim 31 , wherein the cancer is colorectal cancer. 
     
     
         35 . The method of  claim 30 , further comprising administering to the patient in need thereof a therapeutically effective amount of one or more additional pharmaceutically active compounds. 
     
     
         36 . The method of  claim 35 , wherein the one or more additional pharmaceutically active compounds is an anti-PD-1 antibody. 
     
     
         37 . The method of  claim 36 , wherein the anti-PD-1 antibody is pembrolizumab. 
     
     
         38 . The method of  claim 36 , wherein the anti-PD-1 antibody is niolumab. 
     
     
         39 . The method of  claim 36 , wherein the anti-PD-1 antibody is AMG 404. 
     
     
         40 . The method of  claim 35 , wherein the one or more additional pharmaceutically active compounds is a MEK inhibitor. 
     
     
         41 . The method of  claim 35 , wherein the one or more additional pharmaceutically active compounds is daratumumab. 
     
     
         42 . The method of  claim 35 , wherein the one or more additional pharmaceutically active compounds is an immunomodulatory agent. 
     
     
         43 . Use of a compound according to  claim 1  for treating cancer in a subject. 
     
     
         44 . A compound according to  claim 1  in the preparation of a medicament for treating cancer. 
     
     
         45 . The compound according to  claim 44 , wherein the cancer is non-small cell lung cancer.

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