Encapsulated intestinal midgut endoderm cells

Abstract

Cell populations of intestinal midgut endoderm cells and methods of generating the cells expressing markers characteristic of intestinal endoderm lineage are disclosed. Methods of treating conditions such as diabetes are also disclosed.

1 . A population of substantially intestinal midgut endoderm cells, wherein the population of cells is encapsulated. 
     
     
         2 . The population of substantially intestinal midgut endoderm cells of  claim 1 , wherein the population of cells is micro-encapsulated. 
     
     
         3 . The population of substantially intestinal midgut endoderm cells of  claim 1 , wherein the population cells is macro-encapsulated. 
     
     
         4 . The population of substantially intestinal midgut endoderm cells of  claim 1 , wherein the intestinal midgut endoderm cells express CDX2 and FOXA2. 
     
     
         5 . The population of substantially intestinal midgut endoderm cells of  claim 1 , wherein the intestinal midgut endoderm cells express transcription factors selected from the group consisting of SOX9, PDX1, KLF5 and HOXC5. 
     
     
         6 . The population of substantially intestinal midgut endoderm cells of  claim 1 , wherein the intestinal midgut endoderm cells do not express transcription factors selected from the group consisting of SOX2, ALB, PTF1A, HOXA13 and LGR5. 
     
     
         7 . The population of substantially intestinal midgut endoderm cells of  claim 1 , wherein the intestinal midgut endoderm cells do not express HAND1. 
     
     
         8 . The population of substantially intestinal midgut endoderm cells of  claim 1 , wherein the population comprises greater than 70% intestinal midgut endoderm cells. 
     
     
         9 . The population of substantially intestinal midgut endoderm cells of  claim 8 , wherein the population comprises greater than 80% intestinal midgut endoderm cells. 
     
     
         10 . The population of substantially intestinal midgut endoderm cells of  claim 8 , wherein the population comprises greater than 90% intestinal midgut endoderm cells. 
     
     
         11 . The population of substantially intestinal midgut endoderm cells of  claim 1 , wherein the population comprises less than 20% mesenchymal cells. 
     
     
         12 . The population of substantially intestinal midgut endoderm cells of  claim 11 , wherein the population comprises less than 10% mesenchymal cells. 
     
     
         13 . The population of substantially intestinal midgut endoderm cells of  claim 11 , wherein the population comprises less than 1% mesenchymal cells. 
     
     
         14 . The population of substantially intestinal midgut endoderm cells of  claim 1 , wherein the population does not include mesenchymal cells.