Bovine milk oligosaccharides

Abstract

Oligosaccharides from bovine milk, whey and dairy products, and methods of producing bovine milk oligosaccharides are provided.

1 . A composition comprising galacto-oligosaccharides (GOS) and/or fructooligosaccharides (FOS) and/or lactulose and/or lactitol and/or xylooligosaccharides and an oligosaccharide from bovine milk or milk product and an inoculum of  Bifidobacterium longum  subsp.  infantis  or  B. breve , wherein the oligosaccharide is selected from the group consisting of:
 an oligosaccharide consisting of 3 Hexose (Hex) moieties, 4 n-acetyl hexosamine (HexNAc) moieties and 1 fucose (Fuc) moiety;   an oligosaccharide consisting of 4 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 3 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 5 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 4 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 3 Hex moieties, 6 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 3 Hex moieties and 6 HexNAc moieties,   an oligosaccharide consisting of 4 Hex moieties and 3 HexNAc moieties;   an oligosaccharide consisting of 3 Hex moieties and 4 HexNAc moieties;   an oligosaccharide consisting of 6 Hex moieties and 2 HexNAc moieties;   an oligosaccharide consisting of 4 Hex moieties and 4 HexNAc moieties;   an oligosaccharide consisting of 3 Hex moieties and 5 HexNAc moieties;   an oligosaccharide consisting of 5 Hex moieties and 4 HexNAc moieties;   an oligosaccharide consisting of 4 Hex moieties and 5 HexNAc moieties; and   a mixture thereof.   
     
     
         2 . The composition of  claim 1  comprising at least two oligosaccharides, wherein the at least two oligosaccharide are selected from the group consisting of:
 an oligosaccharide consisting of 3 Hexose (Hex) moieties, 4 n-acetyl hexosamine (HexNAc) moieties and 1 fucose (Fuc) moiety; 
 an oligosaccharide consisting of 4 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety; 
 an oligosaccharide consisting of 3 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety; 
 an oligosaccharide consisting of 5 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety; 
 an oligosaccharide consisting of 4 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety; 
 an oligosaccharide consisting of 3 Hex moieties, 6 HexNAc moieties, and 1 Fuc moiety; 
 an oligosaccharide consisting of 3 Hex moieties and 6 HexNAc moieties, 
 an oligosaccharide consisting of 4 Hex moieties and 3 HexNAc moieties; 
 an oligosaccharide consisting of 3 Hex moieties and 4 HexNAc moieties; 
 an oligosaccharide consisting of 6 Hex moieties and 2 HexNAc moieties; 
 an oligosaccharide consisting of 4 Hex moieties and 4 HexNAc moieties; 
 an oligosaccharide consisting of 3 Hex moieties and 5 HexNAc moieties; 
 an oligosaccharide consisting of 5 Hex moieties and 4 HexNAc moieties; 
 an oligosaccharide consisting of 4 Hex moieties and 5 HexNAc moieties. 
 
     
     
         3 . The composition of  claim 1 , further comprising a bovine milk protein, a soy protein, betalactoglobulin, whey, soybean oil or starch. 
     
     
         4 . The composition of  claim 2 , further comprising a bovine milk protein, a soy protein, betalactoglobulin, whey, soybean oil or starch. 
     
     
         5 . The composition of  claim 1 , further comprising a non-milk lipid. 
     
     
         6 . The composition of  claim 2 , further comprising a non-milk lipid. 
     
     
         7 . The composition of  claim 1 , wherein the composition is a solid. 
     
     
         8 . The composition of  claim 5 , wherein the composition is a liquid and the content of the oligosaccharide in the composition is from 0.0001-100 g/L. 
     
     
         9 . A method of obtaining one or more of the oligosaccharides recited in  claim 1 , comprising purifying the oligosaccharide from bovine milk and further comprising mixing the oligosaccharide with GOS and/or FOS and/or lactulose and/or lactitol and/or xylooligosaccharides. 
     
     
         10 . A method of treating a condition in an individual comprising administering an amount of a composition to the individual, wherein the composition comprises an oligosaccharide composition from bovine milk or milk product and an inoculum of  Bifidobacterium longum  subsp.  infantis:    an oligosaccharide in the oligosaccharide composition is selected from the group consisting of:   an oligosaccharide consisting of 3 Hexose (Hex) moieties, 4 n-acetyl hexosamine (HexNAc) moieties and 1 fucose (Fuc) moiety;   an oligosaccharide consisting of 4 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 3 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 5 Hex moieties, 4 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 4 Hex moieties, 5 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 3 Hex moieties, 6 HexNAc moieties, and 1 Fuc moiety;   an oligosaccharide consisting of 3 Hex moieties and 6 HexNAc moieties,   an oligosaccharide consisting of 4 Hex moieties and 3 HexNAc moieties;   an oligosaccharide consisting of 3 Hex moieties and 4 HexNAc moieties;   an oligosaccharide consisting of 6 Hex moieties and 2 HexNAc moieties;   an oligosaccharide consisting of 4 Hex moieties and 4 HexNAc moieties;   an oligosaccharide consisting of 3 Hex moieties and 5 HexNAc moieties;   an oligosaccharide consisting of 5 Hex moieties and 4 HexNAc moieties;   an oligosaccharide consisting of 4 Hex moieties and 5 HexNAc moieties; and   a mixture thereof, wherein the oligosaccharide composition and the inoculum of  Bifidobacterium longum  subsp.  infantis  are administered in conjunction or the inoculum of  Bifidobacterium longum  subsp.  infantis  is included in the oligosaccharide composition.   
     
     
         11 . The method of  claim 10 , wherein the method prevents necrotising enterocolitis in the individual. 
     
     
         12 . The method of  claim 10 , wherein the individual is an infant of less than three months of age. 
     
     
         13 . The method of  claim 11 , wherein the individual is a preterm infant.