Maize event dp-004114-3 and methods for detection thereof

Abstract

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

What is claimed is: 
     
         1 . An amplicon comprising the nucleic acid sequence selected from the group consisting of SEQ ID NO: 32, SEQ ID NO: 35 and full length complements thereof. 
     
     
         2 . A biological sample derived from corn event DP-004114-3 plant, tissue, or seed, wherein said sample comprises a nucleotide sequence which is or is complementary to a sequence selected from the group consisting of SEQ ID NO: 32 and SEQ ID NO: 35, wherein said nucleotide sequence is detectable in said sample using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said corn event DP-004114-3 seed of has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506. 
     
     
         3 . The biological sample of  claim 2 , wherein said biological sample comprise plant, tissue, or seed of transgenic corn event DP-004114-3. 
     
     
         4 . The biological sample of  claim 3 , wherein said biological sample is a DNA sample extracted from the transgenic corn plant event DP-004114-3, and wherein said DNA sample comprises one or more of the nucleotide sequences selected from the group consisting of SEQ ID NO: 32, SEQ ID NO: 35, and the complement thereof. 
     
     
         5 . The biological sample of  claim 4 , wherein said biological sample is selected from the group consisting of corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products. 
     
     
         6 . An extract derived from corn event DP-004114-3 plant, tissue, or seed and comprising a nucleotide sequence which is or is complementary to a sequence selected from the group consisting of SEQ ID NO: 32 and SEQ ID NO: 35, wherein a representative sample of said corn event DP-004114-3 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506. 
     
     
         7 . The extract of  claim 6 , wherein said nucleotide sequence is detectable in said extract using a nucleic acid amplification or nucleic acid hybridization method. 
     
     
         8 . The extract of  claim 7 , wherein said extract comprises plant, tissue, or seed of transgenic corn plant event DP-004114-3. 
     
     
         9 . The extract of  claim 8 , further comprising a composition selected from the group consisting of corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products, wherein said composition comprises a detectable amount of said nucleotide sequence. 
     
     
         10 . A method of determining zygosity of DNA of a corn plant comprising corn event DP-004114-3 in a biological sample comprising:
 (a) contacting said sample with a primer pair selected from the group consisting of SEQ ID NOs: 29 and 30, or a primer pair selected from the group consisting of SEQ ID NOs: 33 and 34, such that
 (1) when used in a nucleic acid amplification reaction comprising corn event DP-004114-3 DNA, produces a first amplicon that is diagnostic for corn event DP-004114-3, and 
 (2) when used in a nucleic acid amplification reaction comprising corn genomic DNA other than DP-004114-3 DNA, produces a second amplicon that is diagnostic for corn genomic DNA other than DP-004114-3 DNA; 
   (b) performing a nucleic acid amplification reaction; and   (c) detecting the amplicons so produced, wherein detection of presence of both amplicons indicates that said sample is heterozygous for corn event DP-004114-3 DNA, wherein detection of only the first amplicon indicates that said sample is homozygous for corn event DP-004114-3 DNA.   
     
     
         11 . A pair of polynucleotide primers comprising a first polynucleotide primer and a second polynucleotide primer, wherein the first polynucleotide primer comprises the nucleotide sequence of SEQ ID NO: 29 and the complement thereof; and the second polynucleotide primer comprises the nucleotide sequence of SEQ ID NO: 30 and the complement thereof, which function together in the presence of a DP-004114-3 DNA template in a sample to produce an amplicon diagnostic for event DP-004114-3. 
     
     
         12 . A kit for detecting nucleic acids that are unique to event DP-004114-3 comprising is a primer pair consisting of SEQ ID NOs: 29 and 30, or SEQ ID NOs: 33 and 34, and the complements thereof, which function as a primer pair in a nucleic acid detection method, and which upon amplification of or hybridization to a target nucleic acid sequence in a sample followed by detection of the amplicon or hybridization to the target sequence, are diagnostic for the presence of nucleic acid sequences unique to event DP-004114-3 in the sample.