US2019309355A1PendingUtilityA1

Spatially Encoded Biological Assays

79
Assignee: PROGNOSYS BIOSCIENCES INCPriority: Apr 5, 2010Filed: Jun 17, 2019Published: Oct 10, 2019
Est. expiryApr 5, 2030(~3.7 yrs left)· nominal 20-yr term from priority
Inventors:Mark S. Chee
B01L 3/502715C40B 30/04C12Q 1/6834C12Q 1/6874G01N 33/5308G01N 2458/10C12Q 1/6841C40B 60/04C12Q 1/6869C12Q 1/6876G01N 33/6845C12Q 1/6809C12Q 1/6837C12Q 1/6804G01N 33/543
79
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Claims

Abstract

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

Claims

exact text as granted — not AI-modified
1 . (canceled) 
     
     
         2 . An array comprising a substrate and a plurality of encoded probes, wherein an encoded probe of the plurality of encoded probes comprises: (i) a binding agent and (ii) a coding tag, wherein the plurality of encoded probes are attached to a plurality of locations on the substrate, and wherein the coding tag of the encoded probe corresponds to a location of the plurality of locations on the substrate. 
     
     
         3 . The array of  claim 2 , wherein the plurality of encoded probes are directly immobilized onto the location on the substrate. 
     
     
         4 . The array of  claim 2 , wherein the plurality of encoded probes are indirectly immobilized onto the location on the substrate. 
     
     
         5 . The array of  claim 4 , wherein the plurality of encoded probes are immobilized onto beads, and wherein the beads are immobilized onto the plurality of locations on the substrate. 
     
     
         6 . The array of  claim 2 , wherein the plurality of encoded probes are indirectly immobilized onto the location on the substrate via linkers. 
     
     
         7 . The array of  claim 2 , wherein the binding agent comprises an oligonucleotide. 
     
     
         8 . The array of  claim 2 , wherein the binding agent selectively binds a biological target. 
     
     
         9 . The array of  claim 8 , wherein the biological target comprises a nucleic acid. 
     
     
         10 . The array of  claim 9 , wherein the nucleic acid comprises a single nucleotide polymorphism. 
     
     
         11 . The array of  claim 9 , wherein the nucleic acid comprises a mutation. 
     
     
         12 . The array of  claim 9 , wherein the nucleic acid is genomic DNA. 
     
     
         13 . The array of  claim 9 , wherein the nucleic acid is mRNA. 
     
     
         14 . The array of  claim 8 , wherein the biological target comprises a protein. 
     
     
         15 . The array of  claim 14 , wherein the binding agent comprises an antibody. 
     
     
         16 . The array of  claim 2 , wherein the locations on the substrate comprises wells or channels. 
     
     
         17 . A substrate comprising an encoded probe, wherein the encoded probe comprises:
 (i) a binding agent and (ii) a coding tag, and wherein the encoded probe is attached to a location on the substrate.   
     
     
         18 . The substrate of  claim 17 , wherein the encoded probe is directly immobilized onto the location on the substrate. 
     
     
         19 . The substrate of  claim 17 , wherein the encoded probe is indirectly immobilized onto the location on the substrate. 
     
     
         20 . The substrate of  claim 19 , wherein the encoded probe is immobilized onto a bead, wherein the bead is immobilized to the location onto the substrate. 
     
     
         21 . The substrate of  claim 17 , wherein the encoded probe is indirectly immobilized onto the location on the substrate via a linker. 
     
     
         22 . The substrate of  claim 17 , wherein the binding agent comprises an oligonucleotide. 
     
     
         23 . The substrate of  claim 17 , wherein the binding agent selectively binds a biological target. 
     
     
         24 . The substrate of  claim 23 , wherein the biological target comprises a nucleic acid. 
     
     
         25 . The substrate of  claim 24 , wherein the nucleic acid comprises a single nucleotide polymorphism. 
     
     
         26 . The substrate of  claim 24 , wherein the nucleic acid comprises a mutation. 
     
     
         27 . The substrate of  claim 24 , wherein the nucleic acid is genomic DNA. 
     
     
         28 . The substrate of  claim 24 , wherein the nucleic acid is mRNA. 
     
     
         29 . The substrate of  claim 23 , wherein the biological target comprises a protein. 
     
     
         30 . The substrate of  claim 29 , wherein the binding agent comprises an antibody. 
     
     
         31 . The substrate of  claim 17 , wherein the location on the substrate comprises a well or a channel.

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