Administration of tailored feedstock to increase antibiotic susceptibility

Abstract

A method for increasing susceptibility of microorganisms to antibiotics includes providing a microorganism; administering a single type of an antibiotic including a nitroimidazole compound to the microorganism; and administering any of an uronic, aldonic, ulosonic, and aldaric feedstock to the microorganism. The feedstock is adapted to promote cell metabolism and antibiotic activation, and inhibit antibiotic inactivation pathways in the microorganism causing increased sensitivity of the microorganism to the nitroimidazole.

1 . A method for increasing susceptibility of microorganisms to antibiotics, the method comprising:
 providing a microorganism;   administering a single type of an antibiotic comprising a nitroimidazole compound to the microorganism; and   administering any of an uronic, aldonic, ulosonic, and aldaric feedstock to the microorganism,   wherein the feedstock is adapted to promote cell metabolism and antibiotic activation, and inhibit antibiotic inactivation pathways in the microorganism causing increased sensitivity of the microorganism to the nitroimidazole.   
     
     
         2 . The method of  claim 1 , wherein the nitroimidazole compound comprise any of metronidazole, tinidazole, ornidazole, nimorazole, secnidazole, azanidazole propenidazole, and derivatives thereof. 
     
     
         3 . The method of  claim 1 , wherein the feedstock comprises a combination of two or more of uronic, aldonic, ulosonic, and aldaric acid. 
     
     
         4 . The method of  claim 1 , wherein the feedstock is adapted to produce adenosine triphosphate (ATP) and reduced ferredoxin, and to decrease production of NADH and NADPH in the microorganism. 
     
     
         5 . (canceled) 
     
     
         6 . The method of  claim 1 , wherein the feedstock is adapted to activate the nitroimidazole compound causing metabolic activation of the microorganism in the microorganism. 
     
     
         7 . The method of  claim 1 , comprising administering the feedstock as a supplement for oral antibiotics to said microorganism or a host thereof. 
     
     
         8 . The method of  claim 1 , wherein the feedstock is administered in an aqueous solution or a solid form. 
     
     
         9 . The method of  claim 1 , comprising decontaminating the microorganism. 
     
     
         10 . The method of  claim 1 , comprising administering the feedstock orally, topically, or by intravenous injection, subcutaneous injection, or intraperitoneal injection to said microorganism or a host thereof. 
     
     
         11 .- 20 . (canceled) 
     
     
         21 . The method of  claim 1 , further comprising adding a salt of any of an uronic, aldonic, ulosonic, and aldaric acid to an aqueous solution, wherein said salt dissociates to become uronic, aldonic, ulosonic, and/or aldaric acid in said aqueous solution. 
     
     
         22 . The method of  claim 21 , wherein said salt comprises gluconate or galacturonate. 
     
     
         23 . An antibiotic composition comprising:
 an antibiotic comprising a nitroimidazole compound; and   any of an uronic, aldonic, ulosonic, and aldaric feedstock or a salt thereof,   wherein the feedstock is adapted to promote cell metabolism and antibiotic activation, and inhibit antibiotic inactivation pathways in a microorganism causing increased sensitivity of the microorganism to the nitroimidazole.   
     
     
         24 . The antibiotic composition of  claim 23 , further comprising a carrier selected from the group consisting of: sterile water, saline, calcium phosphate, gelatin, dextran, agar, pectin, peanut oil, olive oil, sesame oil, water, glycerol monostearate, glycerol distearate, wax, and polymer. 
     
     
         25 . The antibiotic composition of  claim 23 , further comprising a stabilizer. 
     
     
         26 . The antibiotic composition of  claim 25 , wherein the stabilizer comprises thimerosal. 
     
     
         27 . The antibiotic composition of  claim 23 , further comprising: a preservative or chemical stabilizer selected from the group consisting of: casamino acids, gelatin, phenol red, N—Z amine, monopotassium diphosphate, lactalbumin hydrolysate, and dried milk. 
     
     
         28 . The antibiotic composition of  claim 23 , wherein said nitroimidazole compound is in a free base, neutral or salt form. 
     
     
         29 . The antibiotic composition of  claim 28 , wherein said salt form of the nitroimidazole compound is selected from a free carboxyl group or amine group derived from an inorganic or organic base. 
     
     
         30 . The antibiotic composition of  claim 23 , wherein said composition is an aqueous solution or solid form. 
     
     
         31 . The antibiotic composition of  claim 23 , wherein the composition is adapted to be administered orally, topically, or by intravenous injection, subcutaneous injection or intraperitoneal injection.