Pyruvate dehydrogenase variants, a microorganism comprising the same and a method for producing l-amino acid using the same

Abstract

The present disclosure relates to a novel pyruvate dehydrogenase variant, a polynucleotide encoding the pyruvate dehydrogenase variant, a microorganism of the genus Corynebacterium producing L-amino acid, which includes the pyruvate dehydrogenase variant, and a method for producing an L-amino acid using the microorganism.

1 - 14 . (canceled) 
     
     
         15 . A polypeptide having pyruvate dehydrogenase activity, wherein the polypeptide comprises an amino acid sequence having at least a 95% sequence identity to the SEQ ID NO: 1 and comprising at least one amino acid substitutions in a region of amino acids at positions from 190 to 205 of SEQ ID NO:1. 
     
     
         16 . The polypeptide of  claim 15 , wherein the at least one amino acid substitution in the region of amino acids at positions from 190 to 205 of SEQ ID NO: 1 is selected from the group consisting of amino acids at positions 190, 195, 199, and 201 
     
     
         17 . The polypeptide of  claim 15 , wherein the at least one amino acid substitution in the region of amino acids at positions from 190 to 205 of SEQ ID NO: 1 is selected from the group consisting of a substitution at position 190 from glutamic acid to valine (E190V), a substitution at position 195 from glutamine to histidine (Q195H), a substitution at position 199 from proline to serine (P199S), and a substitution at position 201 from tyrosine to alanine (Y201A). 
     
     
         18 . The polypeptide of  claim 15 , wherein the amino acid sequence is selected from the group consisting of SEQ ID NOS: 15, 17, 18, 22, and 24 to 29. 
     
     
         19 . A polynucleotide encoding the pyruvate dehydrogenase variant of  claim 15 . 
     
     
         20 . A vector comprising the polynucleotide of  claim 19 . 
     
     
         21 . A microorganism of the genus  Corynebacterium  producing L-amino acid, wherein the microorganism comprises the pyruvate dehydrogenase variant of  claim 15 . 
     
     
         22 . The microorganism of  claim 21 , wherein the microorganism is  Corynebacterium glutamicum.    
     
     
         23 . A method for producing L-amino acid, comprising:
 (a) culturing the microorganism of the genus  Corynebacterium  of  claim 21  in a medium to produce an L-amino acid; and   (b) recovering the L-amino acid from the cultured microorganism or the medium.