Method of selecting antioxidants for use in topically applied compositions

Abstract

Antioxidant-containing compositions and methods for confirming antioxidant activity of a composition formulated for topical application to skin. Methods for testing a composition for ability to inhibit both ultraviolet radiation-induced lipid peroxidation on skin and ultraviolet radiation-induced reactive oxygen species formation in the stratum corneum. Compositions and methods for treating and preventing photodamage to skin.

1 - 2 . (canceled) 
     
     
         3 . A composition for topical application, wherein the composition comprises at least one antioxidant compound that substantially inhibits ultraviolet radiation-induced reactive oxygen species formation in the upper layers of the epidermis and at least one antioxidant compound that substantially inhibits ultraviolet radiation-induced reactive oxygen species formation in the lower layers of the epidermis. 
     
     
         4 . The composition of  claim 3 , which comprises Vitamin E in an amount of about 0.25% by weight. 
     
     
         5 . The composition of  claim 3 , which comprises Vitamin E in an amount of about 0.5% by weight. 
     
     
         6 . The composition of  claim 3 , which comprises diethylhexyl syringylidene malonate. 
     
     
         7 . The composition of  claim 4 , which further comprises diethylhexyl syringylidene malonate. 
     
     
         8 . The composition of  claim 5 , which further comprises diethylhexyl syringylidene malonate. 
     
     
         9 . The composition of  claim 3 , which further comprises at least one sunscreen active agent. 
     
     
         10 . The composition of  claim 9 , wherein the sunscreen active agent is chosen from the group consisting of para aminobenzoic acid, avobenzone, cinoxate, dioxybenzone, homosalate, menthyl anthranilate, octyl salicylate, oxybenzone, padimate O, phenylbenzimidazole sulfonic acid, sulisobenzone, trolamine salicylate, titanium dioxide, zinc oxide, diethanolamine methoxycinnamate, digalloy trioleate, ethyl dihydroxypropyl PABA, glyceryl aminobenzoate, lawsone with dihydroxyacetone, red petrolatum, ethylhexyl triazone, dioctyl butamido triazone, benzylidene malonate polysiloxane, terephthalylidene dicamphor sulfonic acid, disodium phenyl dibenzimidazole tetrasulfonate, diethylamino hydroxybenzoyl hexyl benzoate, bis diethylamino hydroxybenzoyl benzoate, bis benzoxazoylphenyl ethylhexylimino triazine, drometrizole trisiloxane, methylene bis-benzotriazolyl tetramethylbutylphenol, bis-ethylhexyloxyphenol methoxyphenyltriazine, 4-methylbenzylidenecamphor, isopentyl 4-methoxycinnamate, and mixtures thereof.