Apparatus, systems, and methods for mapping of tissue oxygenation

Abstract

Apparatus, systems, and methods are provided that generate in vivo maps of oxygenation measurements of biological tissue. These may include surgical instruments and stand-alone imaging systems with incorporated oxygen sensing capability. Oxygenation maps can be determined via fluorescent or phosphorescent lifetime imaging of an injectable probe with an oxygen-dependent optical response. Probe configuration and methods and apparatus of injecting the probe into the tissue are provided. Methods and apparatus for temperature compensation of temperature-dependent lifetime measurements are provided to improve oxygenation measurement accuracy. Oxygen maps may be registered with visible light images to assist in assessing tissue viability or localize anomalies in the tissue. Resulting oxygen images may be used for various applications including, but not limited to, guiding surgical procedures such as colorectal resection through use of intraoperative sensing, enhanced endoscopic imaging for identifying suspect lesions during colonoscopy, and external imaging of tissue such as assessing peripheral vascular disease.

1 . An imaging system that resolves and maps a physiologic condition, or proxy thereof; the imaging system utilizing information obtained from two or more sensing modalities to resolve said physiologic condition or proxy thereof; the sensing modalities used in conjunction provide improved accuracy of absolute measurements of said physiologic condition or measurement. 
     
     
         2 .- 20 . (canceled)