Microorganism having acyltransferase activity and uses thereof

Abstract

The present disclosure relates to a polypeptide having an acyltransferase activity or a microorganism including the same; a composition for preparing N-acetyl-L-methionine, the composition including the polypeptide or microorganism; and a method of preparing N-acetyl-L-methionine using the polypeptide or microorganism. Further, the present disclosure relates to a polynucleotide encoding the polypeptide and an expression vector including the polynucleotide. Since the microorganism including a novel acyltransferase according to the present disclosure has enhanced acyltransferase activity, this microorganism can be efficiently used for producing N-acetyl-L-methionine by acetylating L-methionine.

1 . A microorganism having an acyltransferase activity, the microorganism comprising a polypeptide represented by an amino acid sequence of any one of SEQ ID NOS. 1 to 6 or an amino acid sequence having 90% or more homology to the amino acid sequence. 
     
     
         2 . The microorganism according to  claim 1 , wherein the microorganism is selected from the group consisting of  Escherichia  sp.,  Corynebacterium  sp.,  Saccharomyces  sp., and  Yarrowia  sp. 
     
     
         3 . The microorganism according to  claim 1 , wherein the microorganism has an acetyltransferase activity to L-methionine and thus produces N-acetyl-L-methionine. 
     
     
         4 . A polypeptide having an acetyltransferase activity, the polypeptide being represented by an amino acid sequence of any one of SEQ ID NOS. 1 to 6 or an amino acid sequence having 90% or more homology to the amino acid sequence. 
     
     
         5 . A polynucleotide encoding the polypeptide of  claim 4 . 
     
     
         6 . An expression vector comprising the polynucleotide of  claim 5 . 
     
     
         7 . A composition for preparing N-acetyl-L-methionine from L-methionine, the composition comprising, as an active ingredient: (i) the polypeptide of  claim 4 ; (ii) a microorganism comprising the polypeptide or a culture of the microorganism; or a combination thereof. 
     
     
         8 . A method of preparing N-acetyl-L-methionine, comprising:
 acetylating L-methionine using (i) the polypeptide of  claim 4 ; (ii) a microorganism comprising the polypeptide or a culture of the microorganism; or a combination thereof.   
     
     
         9 . The method according to  claim 8 , further comprising:
 recovering N-acetyl-L-methionine, which is the acetylated L-methionine.   
     
     
         10 . The composition of  claim 7 , wherein the microorganism is selected from the group consisting of  Escherichia  sp.,  Corynebacterium  sp.,  Saccharomyces  sp., and  Yarrowia  sp. 
     
     
         11 . The composition of  claim 7 , wherein the microorganism has an acetyltransferase activity to L-methionine and thus produces N-acetyl-L-methionine. 
     
     
         12 . The method of  claim 8 , wherein the microorganism is selected from the group consisting of  Escherichia  sp.,  Corynebacterium  sp.,  Saccharomyces  sp., and  Yarrowia  sp. 
     
     
         13 . The method of  claim 8 , wherein the microorganism has an acetyltransferase activity to L-methionine and thus produces N-acetyl-L-methionine. 
     
     
         14 . The method of  claim 12 , further comprising:
 recovering N-acetyl-L-methionine, which is the acetylated L-methionine.   
     
     
         15 . The method of  claim 13 , further comprising:
 recovering N-acetyl-L-methionine, which is the acetylated L-methionine.