Method for producing immobilized allulose epimerase

Abstract

The purpose of the present invention is to provide a method for efficiently producing an immobilized allulose epimerase having high activity and excellent durability. An immobilized allulose epimerase having high specific activity and excellent durability can be produced efficiently by bringing an enzyme solution containing an allulose epimerase having a specific activity equal to or higher than a specific level into contact with a styrene-based porous-type weakly basic anion exchange resin or a styrene-based gel-type weakly basic anion exchange resin.

1 . A method for producing an immobilized allulose epimerase comprising a step of bringing an enzyme solution containing allulose epimerase having a specific activity of 50 U/mg or more into contact with a styrene-based porous-type weakly basic anion exchange resin or a styrene-based gel-type weakly basic anion exchange resin such that the total loaded protein amount is 1.3 to 15 mg/ml-R. 
     
     
         2 . The method according to  claim 1 , wherein an ion exchange group of the styrene-based porous-type weakly basic anion exchange resin or the styrene-based gel-type weakly basic anion exchange is a tertiary amine. 
     
     
         3 . The method according to  claim 1 , wherein an ion exchange group of the styrene-based porous-type weakly basic anion exchange resin is —N(CH 3 ) 2 . 
     
     
         4 . The method according to of  claim 1 , wherein the number of allulose epimerase units of the enzyme solution to be brought into contact with the ion exchange resin is 270 U or more per 1 ml of the ion exchange resin.