Platelet Products and Storage Media for Platelet Concentrates

Abstract

Automated systems and methods for providing platelet concentrates and synthetic storage media with reduced residual plasma volumes are disclosed. The disclosed systems and methods reduce the residual volume of plasma in platelet concentrate to obtain a platelet product having a volume of plasma that is approximately 5% or less of the total platelet product volume. The disclosed systems and methods also reduce the residual volume of plasma in platelet concentrate to obtain a washed platelet product, wherein the volume of plasma in the washed platelet product is approximately 1% or less of the total washed platelet product volume. Storage media for platelets including less than approximately 10% plasma are also disclosed.

1 . A synthetic aqueous solution for the storage of platelets comprising:
 from about 45 to about 125 mM sodium chloride;   from about 5 to about 15 mM sodium citrate;   from about 20 to about 40 mM sodium acetate;   from about 0.05 to about 12 mM phosphate;   from about 0.05 to about 3 mM magnesium ion;   from about 0.05 to about 10 mM potassium chloride;   from about 5 to about 40 mM sodium bicarbonate; and   from about 0.5 to about 30 mM glucose.   
     
     
         2 . The aqueous solution of  claim 1  further comprising from about 0 mM to 3 mM calcium chloride. 
     
     
         3 . A platelet product, comprising:
 platelets, and
 an aqueous solution comprising:
 from about 45 to about 120 mM sodium chloride; 
 from about 5 to about 15 mM sodium citrate; 
 from about 20 to about 40 mM sodium acetate; 
 from about 0.05 to about 12 mM sodium phosphate; 
 from about 0.05 to about 3 mM magnesium ion; 
 from about 0.05 to about 10 mM potassium chloride; 
 from about 5 to about 40 mM sodium bicarbonate; 
 from about 0.5 to about 30 mM glucose; and 
 up to about 10% plasma. 
 
   
     
     
         4 . The platelet product of  claim 3  further comprising from about 0 mM to 3 mM calcium chloride. 
     
     
         5 . The platelet product of  claim 3  wherein the aqueous solution comprises up to about 5% plasma. 
     
     
         6 . The platelet product of  claim 3  wherein the aqueous solution comprises up to about 1% plasma. 
     
     
         7 . A method of storing platelets, comprising:
 (a) providing an amount of platelets;   (b) combining said platelets with a volume of an aqueous solution, said solution comprising:
 from about 45 to about 125 mM sodium chloride; 
 from about 5 to about 15 mM sodium citrate; 
 from about 20 to about 40 mM sodium acetate; 
 from about 0.05 to about 12 mM phosphate; 
 from about 0.05 to about 3 mM magnesium ion; 
 from about 0.05 to about 10 mM potassium chloride; 
 from about 5 to about 40 mM sodium bicarbonate; and 
 from about 0.5 to about 30 mM glucose; and 
 up to about 10% plasma. 
   
     
     
         8 . The method of  claim 7  wherein the aqueous solution further comprises from about 0 to 3 mM calcium chloride. 
     
     
         9 . The method of  claim 7  wherein the aqueous solution comprises less than 10% plasma. 
     
     
         10 . The method of  claim 7  wherein the aqueous solution comprises less than 5% plasma. 
     
     
         11 . The method of  claim 7  wherein the aqueous solution comprises less than 1% plasma. 
     
     
         12 . The method of  claim 7  further comprising storing the platelets for up to 9 days. 
     
     
         13 . The method of  claim 7  further comprising storing platelets for up to 14 days. 
     
     
         14 . An aqueous solution for the storage of platelets comprising:
 about 69 mM sodium chloride;   about 10 mM sodium citrate;   about 30 mM sodium acetate;   about 9.3 mM phosphate monobasic dihydrate and dibasic sodium phosphate anhydrous;   about 1.5 mM magnesium chloride;   about 5 mM potassium chloride;   about 16.8 mM glucose (dextrose); and   at least about 10 mM sodium bicarbonate.   
     
     
         15 . The aqueous solution of  claim 14  comprising about 20 mM of sodium bicarbonate. 
     
     
         16 .- 33 . (canceled)