Medium, additive for albumin-free medium, and method for culturing pluripotent stem cells

Abstract

An object of the present invention is to provide an albumin-free medium for serum-free culture of pluripotent stem cells capable of maintaining the undifferentiated state and the pluripotency (pluripotent ability) of the pluripotent stem cells, an additive for an albumin-free medium, and a culture method. The albumin-free medium for serum-free culture of pluripotent stem cells, the additive for an albumin-free medium, and a medium used for the culture method of the invention are characterized by containing at least one selected from the group consisting of a Pluronic nonionic surfactant, an animal-based hydrolysate, and a nonanimal-based hydrolysate.

1 . A medium for serum-free culture of pluripotent stem cells, containing at least one selected from the group consisting of a Pluronic nonionic surfactant, an animal-based hydrolysate, and a nonanimal-based hydrolysate, and not containing albumin. 
     
     
         2 . The medium according to  claim 1 , further containing at least one of a lipid mixture and water-soluble oleic acid. 
     
     
         3 . The medium according to  claim 2 , wherein the Pluronic nonionic surfactant is Pluronic (registered trademark) F-68. 
     
     
         4 . The medium according to  claim 3 , wherein the nonanimal-based hydrolysate is EX-CELL™ CD Hydrolysate Fusion. 
     
     
         5 . An additive for an albumin-free medium for serum-free culture of pluripotent stem cells, containing at least one selected from the group consisting of a Pluronic nonionic surfactant, an animal-based hydrolysate, and a nonanimal-based hydrolysate. 
     
     
         6 . The additive for an albumin-free medium according to  claim 5 , further containing at least one of a lipid mixture and water-soluble oleic acid. 
     
     
         7 . The additive for an albumin-free medium according to  claim 6 , wherein the nonanimal-based hydrolysate is EX-CELL™ CD Hydrolysate Fusion. 
     
     
         8 . A method for culturing pluripotent stem cells, comprising culturing pluripotent stem cells in a serum-free medium containing at least one selected from the group consisting of a Pluronic nonionic surfactant, an animal-based hydrolysate, and a nonanimal-based hydrolysate, and not containing albumin, thereby proliferating the pluripotent stem cells while maintaining the pluripotency of the pluripotent stem cells. 
     
     
         9 . The culture method according to  claim 8 , wherein the serum-free medium further contains at least one of a lipid mixture and water-soluble oleic acid. 
     
     
         10 . The culture method according to  claim 9 , wherein the nonanimal-based hydrolysate is EX-CELL™ CD Hydrolysate Fusion. 
     
     
         11 . The culture method according to  claim 8 , wherein the nonanimal-based hydrolysate is EX-CELL™ CD Hydrolysate Fusion. 
     
     
         12 . The medium according to  claim 1 , wherein the Pluronic nonionic surfactant is Pluronic (registered trademark) F-68. 
     
     
         13 . The medium according to  claim 12 , wherein the nonanimal-based hydrolysate is EX-CELL™ CD Hydrolysate Fusion. 
     
     
         14 . The medium according to  claim 2 , wherein the nonanimal-based hydrolysate is EX-CELL™ CD Hydrolysate Fusion. 
     
     
         15 . The medium according to  claim 1 , wherein the nonanimal-based hydrolysate is EX-CELL™ CD Hydrolysate Fusion. 
     
     
         16 . The additive for an albumin-free medium according to  claim 5 , wherein the nonanimal-based hydrolysate is EX-CELL™ CD Hydrolysate Fusion.