Mesenchymal stromal cells and uses related thereto

Abstract

The present invention generally relates to novel preparations of mesenchymal stromal cells (MSCs) derived from hemangioblasts, methods for obtaining such MSCs, and methods of treating a pathology using such MSCs. The methods of the present invention produce substantial numbers of MSCs having a potency-retaining youthful phenotype, which are useful in the treatment of pathologies.

1 . A pharmaceutical preparation suitable for use in a mammalian patient, comprising at least 10 6  mesenchymal stromal cells and a pharmaceutically acceptable carrier, wherein the mesenchymal stromal cells have replicative capacity to undergo (a) at least 10 population doublings in cell culture with less than 25 percent of the cells undergoing cell death, senescing or differentiating into non-MSC cells by the tenth doubling, or (b) at least 5 passages in cell culture with less than 25 percent of the cells undergoing cell death, senescing or differentiating into fibroblasts by the 5th passage. 
     
     
         2 . (canceled) 
     
     
         3 . A pharmaceutical preparation comprising at least 10 6  mesenchymal stromal cells and a pharmaceutically acceptable carrier, wherein the mesenchymal stromal cells are differentiated from a hemangioblast cell. 
     
     
         4 . A cryogenic cell bank comprising at least 10 6  mesenchymal stromal cells, wherein the mesenchymal stromal cells have replicative capacity to undergo at least 10 population doublings in cell culture with less than 25 percent of the cells undergoing cell death, senescing or differentiating into fibroblasts by the tenth population doubling. 
     
     
         5 .- 45 . (canceled) 
     
     
         46 . A method for generating mesenchymal stromal cells of the preparation of  claim 2  comprising culturing hemangioblasts under conditions that give rise to mesenchymal stem cells. 
     
     
         47 .- 82 . (canceled) 
     
     
         83 . Mesenchymal stromal cells derived from hemangioblasts obtained by the method of  claim 46 . 
     
     
         84 .- 103 . (canceled) 
     
     
         104 . A kit comprising the preparation of mesenchymal stromal cells of  claim 1 , wherein said cells or preparation of cells are frozen or cryopreserved, or wherein said cells or preparation of cells is contained in a cell delivery vehicle. 
     
     
         105 . (canceled) 
     
     
         106 . A method for treating a disease or disorder, comprising administering an effective amount of the preparation of mesenchymal stromal cells according to  claim 1  to a subject in need thereof. 
     
     
         107 - 112 . (canceled) 
     
     
         113 . A method of treating bone loss or cartilage damage comprising administering an effective amount of the preparation of mesenchymal stromal cells according to  claim 1  to a subject in need thereof. 
     
     
         114 - 125 . (canceled)