Antimicrobial compositions and methods of use thereof

Abstract

The present application discloses a method for treating microbial infection using an antimicrobial composition comprises antimicrobial peptide which contains at least one VGFPV motif.

1 . A method of treating a microbial infection, comprising:
 administering to a subject in need thereof an effective amount of an antimicrobial peptide comprising a first antimicrobial domain comprising at least two VGVSV (SEQ ID NO:2) motifs.   
     
     
         2 . The method of  claim 1 , wherein said antimicrobial peptide has a length of 10-200 amino acids. 
     
     
         3 . (canceled) 
     
     
         4 . The method of  claim 1 , wherein said antimicrobial peptide comprises at least one VGVSV motif at the N-terminal. 
     
     
         5 . The method of  claim 1 , wherein said antimicrobial peptide comprises at least one VGVSV motif at the C-terminal. 
     
     
         6 . The method of  claim 1 , wherein said antimicrobial peptide comprises the amino acid sequence of VGVSVAAVGVSV (SEQ ID NO:4). 
     
     
         7 . The method of  claim 1 , wherein said antimicrobial peptide further comprises a second antimicrobial domain, wherein said second antimicrobial domain is different from said first antimicrobial domain. 
     
     
         8 . The method of  claim 1 , wherein said antimicrobial peptide further comprises a cell penetrating peptide (CPP) domain. 
     
     
         9 . The method of  claim 1 , wherein said antimicrobial peptide further comprises a targeting domain. 
     
     
         10 . The method of  claim 1 , wherein said antimicrobial peptide is administered in combination with another antimicrobial agent. 
     
     
         11 . The method of  claim 10 , wherein said another antimicrobial agent is another antimicrobial peptide. 
     
     
         12 . The method of  claim 10 , wherein said another antimicrobial agent is an in vivo expression vector encoding said antimicrobial peptide. 
     
     
         13 . The method of  claim 10 , wherein said antimicrobial peptide is a PEGylated peptide. 
     
     
         14 - 18 . (canceled) 
     
     
         19 . A pharmaceutical composition for treating microbial infection, comprising:
 (1) an antimicrobial peptide comprising a first antimicrobial domain comprising two VGVSV (SEQ ID NO:2) motifs, and a cell penetrating peptide (CPP) domain and/or a targeting domain; and   (2) a pharmaceutically acceptable carrier.   
     
     
         20 . The pharmaceutical composition of  claim 19 , wherein said antimicrobial peptide is a PEGylated peptide. 
     
     
         21 . The pharmaceutical composition of  claim 19 , wherein said antimicrobial peptide further comprises a second antimicrobial domain, wherein said second antimicrobial domain is different from said first antimicrobial domain.