US2019142916A1PendingUtilityA1

Biomarkers and immunogenic compositions for filarial parasites

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Assignee: NEW YORK BLOOD CENTER INCPriority: Apr 1, 2016Filed: Mar 31, 2017Published: May 16, 2019
Est. expiryApr 1, 2036(~9.7 yrs left)· nominal 20-yr term from priority
Inventors:Sara LustigmanThomas B. NutmanSasisekhar Bennuru
C07K 14/4354A61K 2039/552C07K 16/18G01N 33/5308A61K 39/0003A61P 33/10A61K 2039/6031A61K 2039/55505A61K 2039/70
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Claims

Abstract

Disclosed herein are immunogenic compositions for preventing or treating infection with filarial parasites and biomarkers for diagnosing infection with filarial parasites.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . An immunogenic composition for preventing or treating infection with a filarial parasite, wherein the filarial parasite is  Onchocerca volvulus , and wherein the immunogenic composition comprises at least one filarial parasite protein having at least 85% sequence identity to full length mature protein OVOC8619 (SEQ ID NO:16), OVOC7083 (SEQ ID NO:17), OVOC4111 (SEQ ID NO:18), OVOC1808 (SEQ ID NO:19), OVOC11598 (SEQ ID NO:20), OVOC3901 (SEQ ID NO:21), OVOC10819 (SEQ ID NO:22), OVOC5395 (SEQ ID NO:23), OVOC12235 (SEQ ID NO:24), OVOC7908 (SEQ ID NO:25), OVOC7430 (SEQ ID NO:26), OVOC8936 (SEQ ID NO:27), OVOC5806 (SEQ ID NO:28), OVOC4665 (SEQ ID NO:29), or OVOC8227 (SEQ ID NO:30). 
     
     
         2 . An immunogenic composition for preventing or treating infection with a filarial parasite, wherein the filarial parasite is  Dirofilaria immitis , and wherein the immunogenic composition comprises at least one filarial parasite protein having at least 85% sequence identity to full length mature protein of OVOC8619 (SEQ ID NO:16), OVOC7083 (SEQ ID NO:17), OVOC4111 (SEQ ID NO:18), OVOC1808 (SEQ ID NO:19), OVOC11598 (SEQ ID NO:20), OVOC3901 (SEQ ID NO:21), OVOC10819 (SEQ ID NO:22), OVOC5395 (SEQ ID NO:23), OVOC12235 (SEQ ID NO:24), OVOC7908 (SEQ ID NO:25), OVOC7430 (SEQ ID NO:26), OVOC8936 (SEQ ID NO:27), OVOC5806 (SEQ ID NO:28), OVOC4665 (SEQ ID NO:29), OVOC8227 (SEQ ID NO:30), OVOC9988 (SEQ ID NO:31), or OVOC4230 (SEQ ID NO:32), or an ortholog thereof. 
     
     
         3 . The immunogenic composition of  claim 2 , wherein the ortholog comprises a filarial parasite protein having at least 85% sequence identity to the full length of SEQ ID NO:33-49. 
     
     
         4 . The immunogenic composition of either of  claim 1 , wherein the immunogenic composition further comprises an adjuvant. 
     
     
         5 . The immunogenic composition of either of  claim 1 , wherein the immunogenic composition comprises at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, or at least ten filarial parasite proteins. 
     
     
         6 . The immunogenic composition of  claim 5 , wherein the at least two filarial parasite proteins are present in the immunogenic composition as a mixture. 
     
     
         7 . The immunogenic composition of  claim 5 , wherein the at least two filarial parasite proteins are present in the immunogenic composition as a fusion protein comprising the amino acid sequences of the at least two filarial parasite proteins. 
     
     
         8 . The immunogenic composition of  claim 7 , wherein the fusion protein optionally further comprises at least one linker sequence separating the at least two filarial parasite amino acid sequences. 
     
     
         9 . A method of preventing infection with, or transmission of,  O. volvulus , the method comprising administering an immunogenic composition of  claim 1  to a subject in need thereof, wherein the immunogenic composition prevents or treats the infection. 
     
     
         10 . The method of  claim 9 , wherein the immunogenic composition is administered to a subject at risk of  O. volvulus  infection, and the administration prevents infection with  O. volvulus  and/or prevents transmission of  O. volvulus.    
     
     
         11 . The method of  claim 9 , wherein the subject is a human. 
     
     
         12 . A method of preventing an infection with  D. immitis , the method comprising administering an immunogenic composition of  claim 2  to a canine subject in need thereof, wherein the immunogenic composition prevents or treats the infection. 
     
     
         13 . The method of  claim 12 , wherein the immunogenic composition is administered to a subject at risk of  D. immitis  infection, and the administration prevents infection with  D. immitis.    
     
     
         14 . A method of detecting infection with  O. volvulus , comprising identifying in a specimen from a subject at least one filarial protein having at least 85% sequence identity to OVOC10469 (SEQ ID NO:1), OVOC11950 (SEQ ID NO:2), OVOC10602 (SEQ ID NO:3), OVOC3261 (SEQ ID NO:4), OVOC5127 (SEQ ID NO:5), OVOC8491 (SEQ ID NO:6), OVOC6759 (SEQ ID NO:7), OVOC451 (SEQ ID NO:8), OVOC12329 (SEQ ID NO:9), OVOC3337 (SEQ ID NO:10), OVOC10264 (SEQ ID NO:11), OVOC4230 (SEQ ID NO:12), OVOC10384 (SEQ ID NO:13), OVOC8422 (SEQ ID NO:14), OVOC9988 (SEQ ID NO:31), or OVOC6395 (SEQ ID NO:15), or an immunoreactive fragment thereof. 
     
     
         15 . The method of  claim 14 , wherein the specimen comprises blood, a skin biopsy, or urine. 
     
     
         16 . The method of  claim 14 , wherein the immunoreactive fragment comprises the amino acid sequence of OVOC10469_Pep2 (SEQ ID NO:51), OVOC3261_Pep1 (SEQ ID NO:52), OVOC3261_Pep3 (SEQ ID NO:53), OVOC10469_Pep1 (SEQ ID NO:54), OVOC10469_Pep3 (SEQ ID NO:55), OVOC3261_Pep2 (SEQ ID NO:56), OVOC5127_Pep1 (SEQ ID NO:57), OVOC5127_Pep2 (SEQ ID NO:58), OVOC5127_Pep4, (SEQ ID NO:59), OVOC5127_Pep5 (SEQ ID NO:60), and OVOC5127_PepX (SEQ ID NO:61). 
     
     
         17 . The method of  claim 14 , wherein the method further comprises detecting at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, or at least ten filarial parasite proteins. 
     
     
         18 . The method of  claim 17 , wherein the at least two filarial parasite proteins comprise Ov16 and OVOC3261. 
     
     
         19 . The method of  claim 17 , wherein the at least four filarial parasite proteins comprise Ov16, OVOC3261, OVOC10469, and OVOC5127. 
     
     
         20 . The method of  claim 14 , wherein the filarial parasite protein is detected by a method selected from the group consisting of ELISA, dipstick tests, lateral flow, microfluidic devices, luciferase immunoprecipitation systems, luminex, multiplex-formats, and microarrays. 
     
     
         21 . A method of detecting infection with  O. volvulus , comprising identifying in the blood of a subject, antibodies to at least one filarial protein having at least 85% sequence identity to OVOC10469 (SEQ ID NO:1), OVOC11950 (SEQ ID NO:2), OVOC10602 (SEQ ID NO:3), OVOC3261 (SEQ ID NO:4), OVOC5127 (SEQ ID NO:5), OVOC8491 (SEQ ID NO:6), OVOC6759 (SEQ ID NO:7), OVOC451 (SEQ ID NO:8), OVOC12329 (SEQ ID NO:9), OVOC3337 (SEQ ID NO:10), OVOC10264 (SEQ ID NO:11), OVOC4230 (SEQ ID NO:12), OVOC10384 (SEQ ID NO:13), OVOC8422 (SEQ ID NO:14), OVOC9988 (SEQ ID NO:31), or OVOC6395 (SEQ ID NO:15), or an immunoreactive fragment thereof. 
     
     
         22 . The method of  claim 21 , wherein the immunoreactive fragment comprises the amino acid sequence of OVOC10469_Pep2 (SEQ ID NO:51), OVOC3261_Pep1 (SEQ ID NO:52), OVOC3261_Pep3 (SEQ ID NO:53), OVOC10469_Pep1 (SEQ ID NO:54), OVOC10469_Pep3 (SEQ ID NO:55), OVOC3261_Pep2 (SEQ ID NO:56), OVOC5127_Pep1 (SEQ ID NO:57), OVOC5127_Pep2 (SEQ ID NO:58), OVOC5127_Pep4, (SEQ ID NO:59), OVOC5127_Pep5 (SEQ ID NO:60), and OVOC5127_PepX (SEQ ID NO:61). 
     
     
         23 . The method of  claim 21 , wherein the method comprises detecting at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, or at least ten filarial parasite proteins. 
     
     
         24 . The method of  claim 23 , wherein the method further comprises detecting antibodies to at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, or at least ten filarial parasite proteins. 
     
     
         25 . The method of  claim 24 , wherein the at least two filarial parasite proteins comprise Ov16 and OVOC3261. 
     
     
         26 . The method of  claim 24 , wherein the at least four filarial parasite proteins comprise Ov16, OVOC3261, OVOC10469, and OVOC5127. 
     
     
         27 . The method of  claim 21 , wherein the filarial protein or antibody to the filarial protein are detected by a method selected from the group consisting of ELISA, dipstick tests, lateral flow, microfluidic devices, luciferase immunoprecipitation systems, luminex, multiplex-formats, and microarrays.

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