Use of gram negative species to treat atopic dermatitis

Abstract

Pharmaceutical compositions are disclosed that includes a therapeutically effective amount of a purified viable gram negative bacteria and a pharmaceutically acceptable carrier. The pharmaceutical compositions are formulated for topical administration. Methods of treating atopic dermatitis using these pharmaceutical compositions are also disclosed.

1 . A method for treatment of atopic dermatitis, comprising:
 topically administering a composition comprising at least one species of gram negative bacteria to a subject in need thereof, wherein the at least one species of gram negative bacteria is present in an amount sufficient for treatment of atopic dermatitis in the subject, and wherein the at least one species of gram negative bacteria is isolated from skin of a subject that does not have atopic dermatitis.   
     
     
         2 . The method of  claim 1 , wherein the at least one species of gram negative bacteria comprises a species of  Pseudomonas.    
     
     
         3 . The method of  claim 2 , wherein the species of  Pseudomonas  is  Pseudomonas aeruginosa, Pseudomonas luteola,  or  Pseudomonas orbyhabitans.    
     
     
         4 . The method of  claim 1 , wherein the at least one species of gram negative bacteria comprises a species of  Pantoea.    
     
     
         5 . The method of  claim 4 , wherein the species of  Pantoea  is  Pantoea septica.    
     
     
         6 . The method of  claim 1 , wherein the at least one species of gram negative bacteria comprises a species of  Moraxella.    
     
     
         7 . The method of  claim 6 , wherein the species of  Moraxella  is  Moraxella osloensis.    
     
     
         8 . The method of  claim 1 , wherein the at least one species of gram negative bacteria comprises a species of  Methylobacterium.    
     
     
         9 . The method of  claim 1 , wherein the at least one species of gram negative bacteria comprises up to five strains of a single species of gram negative bacteria. 
     
     
         10 . The method of  claim 9 , wherein the at least one species of gram negative bacteria comprises up to three strains of a single species of gram negative bacteria. 
     
     
         11 . The method of  claim 9 , wherein the single species of gram negative bacteria is  Acinetobacter radioresistens, Moraxella osloensis, Pantoea septica, Pseudomonas luteola, Pseudomonas aeruginosa,  or  Pseudomonas oryzibhabitns.    
     
     
         12 . The method of  claim 1 , wherein the at least one species of gram negative bacteria is present in an amount of 10 4  to 10 12  colony forming units. 
     
     
         13 . The method of  claim 1 , wherein at least one species of gram negative bacteria is topically administered by spraying. 
     
     
         14 . The method of  claim 1 , wherein the at least one species of gram negative bacteria is topically administered to the subject at least two times per a week. 
     
     
         15 . The method of  claim 1 , wherein the at least one species of gram negative bacteria is topically administered to the subject every other day over a week. 
     
     
         16 . The method of  claim 1 , wherein the at least one species of gram negative bacteria is topically administered to the subject daily over a week. 
     
     
         17 . The method of  claim 1 , wherein the subject is an adult. 
     
     
         18 . The method of  claim 1 , wherein the subject is a child. 
     
     
         19 . The method of  claim 1 , wherein the subject is an infant. 
     
     
         20 . The method of  claim 1 , wherein the at least one species of gram negative bacteria comprises 1, 2, 3, 4, or 5 species of gram negative bacteria. 
     
     
         21 . A method for treatment of atopic dermatitis, comprising: topically administering a composition comprising at least one species of gram negative bacteria to a subject in need thereof, wherein the at least one species of gram negative bacteria is present in an amount sufficient for treatment of atopic dermatitis in the subject, and wherein the at least one species of gram negative bacteria is isolated from skin of a donor subject. 
     
     
         22 . The method of  claim 21 , wherein the at least one species of gram negative bacteria is isolated from a region of the skin of the donor subject which does not comprise eczema.