Three-dimensional optical coherence tomography apparatus and its application

Abstract

Provided herein are devices and systems that apply full-field optical coherence tomography (OCT) technology to three-dimensional skin tissue imaging. A special designed Mirau type objective and an optical microscope module allowing both OCT mode and orthogonal polarization spectral imaging (OPSI) mode are disclosed.

What is claimed is: 
     
         1 . A device comprising a light source module configured to provide a source light to an optical microscope module, which handles the source light and processes light signal; a Mirau type objective module, which handles light from the optical microscope module and process light signal generated from a sample; and a data processing unit for analyzing light signals from the sample, wherein said Mirau type objective module comprises an interference objective immersed in a media, and wherein said optical microscope module comprises an optical switch configured to toggle between optical coherence tomography (OCT) mode and orthogonal polarization spectral imaging (OPSI) mode. 
     
     
         2 . The device of  claim 1 , wherein the light source module comprises an amplified spontaneous emission light source, a superluminescent diode, a light emitting diode (LED), a broadband supercontinuum light source, a mode-locked laser, a tunable laser, a Fourier-domain Mode-locking light source, an optical parametric oscillator (OPO), a halogen lamp, a Ce 3+ :YAG crystal fiber, a Ti 3+ :Al 2 O 3  crystal fiber, or a Cr 4+ :YAG crystal fiber. 
     
     
         3 . The device of  claim 2 , wherein the light source module comprises a Ce 3+ :YAG crystal fiber, Ti 3+ :Al 2 O 3  crystal fiber, or a Cr 4+ :YAG crystal fiber. 
     
     
         4 . The device of  claim 3 , wherein the light source module comprises a Ce 3+ :YAG crystal fiber. 
     
     
         5 . The device of  claim 1 , wherein said Mirau type objective module comprises an interference objective lens immersed in a media, a first glass plate, a second glass plate in a sealed container filled with one or more media. 
     
     
         6 . The device of  claim 1 , wherein the interference objective lens immersed in a media having optical characteristics similar to the tissue sample to be analyzed. 
     
     
         7 . The device of  claim 6 , wherein the optical characteristics is refractive index. 
     
     
         8 . The device of  claim 7 , wherein the media has a refractive index in a range of about 1.2 to about 1.8. 
     
     
         9 . The device of  claim 8 , wherein the media has a refractive index in a range of about 1.3 to about 1.5. 
     
     
         10 . The device of  claim 9 , wherein the media comprises water, silicone oil, ethanol, glycerol, pyrex, a transparent glass or plastic with a refractive index in a range of about 1.3 to about 1.5, or combinations thereof. 
     
     
         11 . The device of  claim 10 , wherein said media comprises water, silicone oil, or glycerol. 
     
     
         12 . The device of  claim 11 , wherein said media comprises silicone oil. 
     
     
         13 . The device of  claim 5 , wherein the one or more media comprises a first media and a second media. 
     
     
         14 . The device of  claim 1 , wherein said first media comprises water and the second media comprises silicone oil. 
     
     
         15 . The device of  claim 1 , wherein the optical switch is a quarter-wave plate where the fast axis of the quarter-wave plate is set to an angle of 45° allowing a portion of a signal light reflected or scattered from the sample passing through with polarization state unchanged. 
     
     
         16 . .The device of  claim 1 , wherein the optical switch is a quarter-wave plate where the fast axis of the quarter-wave plate is set to an angle of 0° or 90° allowing a portion of a signal light reflected or scattered from the sample passing through with polarization state rotated by 90°. 
     
     
         17 . The device of  claim 1 , wherein the optical microscope module further comprises an objective lens, an optical long-wave-pass filter, and a polarization beam splitter. 
     
     
         18 . The device of  claim 1 , wherein the tissue translation module comprises a cover glass and a transversely motorized linear stage on a tissue holder means. 
     
     
         19 . The device of  claim 1 , wherein the light source module comprises a Ce 3+ :YAG crystal fiber, the optical switch is a quarter-wave plate, the Mirau type objective module comprises silicone oil as one or more media. 
     
     
         20 . A system for imaging a sample comprising the device of  claim 1 .