Engineered skin equivalent, method of manufacture thereof and products derived therefrom

Abstract

Disclosed herein are synthetic leathers, artificial epidermal layers, artificial dermal layers, layered structures, products produced therefrom and methods of producing the same.

1 .- 198 . (canceled) 
     
     
         199 . A tanned synthetic leather, which prior to tanning comprises:
 an artificial dermal layer comprising fibroblast, wherein said fibroblast is differentiated from an induced pluripotent stem cell.   
     
     
         200 . The tanned synthetic leather of  claim 199 , which prior to tanning, further comprises an artificial epidermal layer. 
     
     
         201 . The tanned synthetic leather of  claim 200 , wherein said epidermal layer further comprises a keratinocyte. 
     
     
         202 . The tanned synthetic leather of  claim 201 , wherein said keratinocyte is differentiated from an induced pluripotent stem cell. 
     
     
         203 . The tanned synthetic leather of  claim 200 , wherein said epidermal layer is upon said dermal layer thereby forming a layered structure. 
     
     
         204 . The tanned synthetic leather of  claim 203 , wherein said layered structure further comprises a melanocyte. 
     
     
         205 . The tanned synthetic leather of  claim 200 , wherein said epidermal layer further comprises collagen. 
     
     
         206 . The tanned synthetic leather of  claim 199 , wherein said dermal layer further comprises collagen. 
     
     
         207 . The tanned synthetic leather of  claim 199 , wherein said dermal layer is formed upon a scaffold. 
     
     
         208 . The tanned synthetic leather of  claim 207 , wherein said scaffold comprises silk. 
     
     
         209 . The tanned synthetic leather of  claim 208 , wherein said scaffold comprises a natural tissue adhesive. 
     
     
         210 . The tanned synthetic leather of  claim 209 , wherein said natural tissue adhesive comprises fibrin glue. 
     
     
         211 . The tanned synthetic leather of  claim 203 , wherein said synthetic leather further comprises a basement membrane substitute. 
     
     
         212 . The tanned synthetic leather of  claim 211 , wherein said basement membrane substitute is between said epidermal layer and said dermal layer. 
     
     
         213 . The tanned synthetic leather of  claim 212 , wherein said basement membrane substitute comprises a dried acellular amniotic membrane. 
     
     
         214 . The tanned synthetic leather of  claim 199 , comprised in one or more of a watch strap, a belt, a packaging, a shoe, a boot, a footwear, a glove, a clothing, a luggage, a bag, a clutch, a purse, a backpack, a wallet, a saddle, a harness or a whip.