Method and System for Repairing Damaged Tissue Using Nucleated Plasma Particles (Nuc-P2s) and Mesodermal Stem Cells (MesoSCs)

Abstract

The current invention is a method and system for optimally isolating adult derived stem cells from whole blood and reintroducing the adult derived stem cells into a subject at specific target areas having damaged tissue to be repaired.

1 . A method for optimizing the isolation of Mesodermal Stem Cells (MesoSCs) and Nucleated Plasma Particles (Nuc-P2s) for repair of damaged tissue comprising the steps of:
 stimulating the proliferation of MesoSCs and Nuc-P2s in a blood source by administering a predetermined dose of one or more stimulators to the blood source, wherein the blood source is a mammal or non-mammal;   inducing the migration of proliferated MesoSCs and Nuc-P2s into the circulatory system in the blood source by administering a predetermined dose of one or more mobilizers;   administering a predetermined dose of anticoagulant to the blood source;   obtaining a blood sample from the blood source;   administrating a divalent cation chelator to the blood sample to restore MesoSCs′ and Nuc-P2s′ zeta potential;   refrigerating the blood sample to restore active binding sites;   separating MesoSCs and Nuc-P2s from the blood sample; and   separating MesoSCs and sub-types of Nuc-P2s based upon the damaged tissue type and repair desired.   
     
     
         2 . The method of  claim 1  wherein one or more of said stimulators are antioxidants or flavonoids. 
     
     
         3 . The method of  claim 1  wherein the antioxidants are selected from the group consisting essentially of fresh or frozen or powdered blueberries, raspberries, blackberries and concord grapes. 
     
     
         4 . The method of  claim 1  wherein one or more of the mobilizers are selected from the group consisting of a Cyanobacter (Aphanizomenon flos-aquae, AFA), chorella, exercise, and physical trauma. 
     
     
         5 . The method of  claim 1  wherein the divalent cation chelator is selected from the list consisting of, Ethylenediaminetetraacetic acid (EDTA), Ethyleneglycol bis (2-Aminoethyl ether)-N,N,N′,N′ tetraaacetic acid (EGTA), or a calcium chelator equivalent. 
     
     
         6 . A method for treating damaged tissue with optimally isolated Mesodermal Stem Cells (MesoSCs) and Nucleated Plasma Particles (Nuc-P2s) comprising the steps of:
 identifying a recipient having damaged tissue to be treated;   identifying a tissue type to be treated;   determining a route for introduction of optimally isolated MesoSCs and specific Nuc-P2 sub-types to the damaged tissue;   introducing MesoSCs and specific Nuc-P2 sub-types to the damaged tissue; and   introducing MesoSCs and Nuc-P2 sub-types to the recipient systemically.   
     
     
         7 . The method of  claim 6  wherein the optimally isolated of MesoScs and Nuc-P2s is performed by differential centrifugation. 
     
     
         8 . The method of  claim 6  wherein the route of introduction of specific Nuc-P2 sub-types is performed by topical application. 
     
     
         9 . The method of  claim 6  wherein the route of introduction of specific Nuc-P2 sub-types is performed by nebulization. 
     
     
         10 . The method of  claim 6  wherein the route of introduction of specific Nuc-P2 sub-types is performed by direct needle injection with or without fluoroscopic, ultrasound, or other imaging guidance. 
     
     
         11 . The method of  claim 6  wherein the route of introduction of specific Nuc-P2 sub-types is performed by intravenous drip. 
     
     
         12 . The method of  claim 6  wherein the route of introduction of specific Nuc-P2 sub-types is performed by the method of  claim 6  wherein the route of introduction of specific Nuc-P2 sub-types and MesoSCs is accomplished by intravenous drip.