Kit for predicting implantation success in assisted fertilization

Abstract

A method of obtaining a class of embryos having a higher implantation potential than from morphology selection alone by selecting from multiple embryos, obtained by fertilizing the plurality of oocytes, a class of embryos having a high implantation potential. The selection is determined from a level of granulocyte-colony stimulating factor (G-CSF) present in the follicular fluid (FF) of each oocyte in the multiple oocytes.

What is claimed is: 
     
         1 . A method of assisted fertilization, comprising:
 implanting in a subject an embryo, selected from a plurality of embryos obtained by fertilization of a plurality of oocytes collected from the subject, wherein   at least the oocyte having a highest implantation potential of the plurality is implanted as an embryo, and   the implantation potential is determined from a level of granulocyte-colony stimulating factor (G-CSF) present in the follicular fluid (FF) of each oocyte in the plurality of oocytes.   
     
     
         2 . The method of  claim 1 , wherein the level of granulocyte-colony stimulating factor (G-CSF) present in the follicular fluid (FF) of each oocyte in the plurality of oocytes is determined by radio-immunoassay (RIA), enzyme-linked immunosorbent assay (ELISA) or enzyme-linked immunosorbent spot (ELISPOT) assay, surface plasmon resonance, fluorescence resonance energy transfer, bioluminescence resonance energy transfer, fluorescence quenching fluorescence, fluorescence polarization, mass spectrometry (MS), high performance liquid chromatography (HPLC), HPLC/MS, HPLC/MS/MS, capillary electrophoresis, rod or slab gel electrophoresis. 
     
     
         3 . The method of  claim 1 , wherein the oocyte embryo having the highest implantation potential has a level of G-CSF present in the FF which is equal to or greater than 24.0 pg/ml. 
     
     
         4 . The method of  claim 1 , wherein said oocyte having a highest implantation potential has a level of G-CSF present in the FF which is greater than 35.0 pg/ml.