Composition for diagnosing allergies to mites, method of diagnosing allergies to mites, and composition for preventing or treating allergies to mites

Abstract

Mite proteins according to an aspect may be used in diagnosing allergic diseases. Results of diagnosing allergic diseases by using the proteins have high reliability and validity. Further, the proteins may be used in a composition for preventing or treating allergic diseases.

What is claimed is: 
     
         1 . A method of diagnosing an allergic disease to at least one mite species, the method comprising:
 detecting binding of Der f 11, Der f 13, Der f 14, Der f 32, Der f Alt a 10, or a combination thereof and IgE antibodies in a biological sample isolated from a subject;   diagnosing with an allergic disease to at least one mite species when a binding between the IgE antibody and Der f 11, Der f 13, Der f 14, Der f 32, Der f Alt a 10, or a combination thereof is detected,   administrating an effective amount of Der f 11, Der f 13, Der f 14, Der f 32, Der f Alt a 10, or a combination thereof to the subject diagnosed with the allergic disease to at least one mite species.   
     
     
         2 . The method of  claim 1 , wherein the Der f 11, Der f 13, Der f 14, Der f 32, Der f Alt a 10, or a combination thereof comprises an amino acid sequence having about 95% or more sequence homology with any one amino acid sequence selected from SEQ ID NOS: 1, 3, 5, 7, and 9. 
     
     
         3 . The method of  claim 1 , further comprising detecting binding of Der f 1, Der f 2, Der f 10, Der f 30, or a combination thereof and IgE antibodies in the biological sample. 
     
     
         4 . The method of  claim 3 , wherein the Der f 1, Der f 2, Der f 10, Der f 30, or a combination thereof comprises an amino acid sequence having about 95% or more sequence homology with any one amino acid sequence selected from SEQ ID NOS: 11, 13, 15, and 17. 
     
     
         5 . The method of  claim 1 , wherein the detecting is performed by immunoassay. 
     
     
         6 . The method of  claim 1 , wherein the at least one mite species is  Dermatophagoides farinae, Dermatophagoides pteronyssinus, Euroglyphus maynei, Tyrophagus putrescentiae, Blomia tropicalis , or a combination thereof. 
     
     
         7 . The method of  claim 1 , wherein the allergic disease is allergic asthma, allergic rhinitis, atopic dermatitis, or a combination thereof. 
     
     
         8 . The method of  claim 1 , further comprising administrating an effective amount of Der f 1, Der f 2, Der f 10, Der f 30, or a combination thereof to the subject diagnosed with the allergic disease to at least one mite species. 
     
     
         9 . A method of treating an allergic disease to at least one mite species, the method comprising:
 administrating an effective amount of Der f 11, Der f 13, Der f 14, Der f 32, Der f Alt a 10, or a combination thereof to a subject diagnosed with an allergic disease to at least one mite species.   
     
     
         10 . The method of  claim 9 , further comprising administrating an effective amount of Der f 1, Der f 2, Der f 10, Der f 30, or a combination thereof to the subject.