Microalgal compositions and uses thereof

Abstract

Provided are microalgal compositions and methods for their use. The microalgal compositions include lubricants that find use in industrial and other applications.

1 .- 31 . (canceled) 
     
     
         32 . A method for providing lubrication to a surface, the method comprising applying a lubricant to the surface, the lubricant comprising oleaginous microbial biomass, wherein the oleaginous microbial biomass comprises at least 20% lipid by dry cell weight, said lubricant further comprising at least one other ingredient selected from the group consisting of anti-oxidant, corrosion inhibitor, metal deactivator, binder, chelating agent, oxygen scavenger, anti-wear agent, extreme pressure resistance additive, antimicrobial agent, pH adjuster, emulsifier, lubricity agent, vegetable oil, petroleum derived oil, high viscosity petroleum hydrocarbon oil, petroleum derivative, pour point depressant, moisture scavenger, defoamers, anti-misting agent, odorant, surfactant, humectant, rheology modifier, and colorant. 
     
     
         33 . The method of  claim 32 , wherein the oleaginous biomass is unlysed cells and wherein the unlysed cells comprise at least 35% lipid by dry weight. 
     
     
         34 . The method of  claim 32 , wherein the oleaginous biomass comprises at least 50% lipid. 
     
     
         35 . The method of  claim 32 , wherein the antimicrobial agent is selected from the group consisting of 1,2-Benzisothiazolin-3-one, sodium omadine, phenolics, p-chloro-m-cresol, halogen substituted carbamates, isothiazolone derivatives, bromonitriles dinitromorpholines, amphotericin, triazine, BIT, MIT, potassium sorbate, sodium benzoate, and glutaraldehyde. 
     
     
         36 . The method of  claim 35 , wherein the antimicrobial agent is present in the lubricant at a concentration of 0.0005% to 6% by weight. 
     
     
         37 . The method of  claim 32 , wherein the rheology modifier is selected from the group consisting of hydroxyethyl cellulose, carboxymethyl cellulose, xanthan gum, guar gum, starch, and polyanionic cellulose. 
     
     
         38 . The method of  claim 37 , wherein the rheology modifier is present in the lubricant at a concentration of 0.005% to 5% by weight. 
     
     
         39 . The method of  claim 32 , wherein the lipid provided by the oleaginous microbial biomass comprises 15% C16:0 and greater than 55% 18:1. 
     
     
         40 . The method of  claim 32 , wherein the oleaginous microbial biomass is microalgae. 
     
     
         41 . The method of  claim 32 , wherein the microalgae is of the genus  Prototheca, Auxenochlorella, Chlorella , or  Parachlorella.    
     
     
         42 . The method of  claim 41 , wherein the microalgae is of the genus  Prototheca.    
     
     
         43 . The method of  claim 42 , wherein the microalgae is  Prototheca moriformis.    
     
     
         44 . A method for providing lubrication to a surface, the method comprising applying a lubricant to the surface, the lubricant comprising oleaginous microalgal biomass, wherein the oleaginous microalgal biomass is unlysed cells and the unlysed cells comprise at least 20% lipid by dry cell weight, said lubricant further comprising at least one other ingredient selected from the group consisting of anti-oxidant, corrosion inhibitor, metal deactivator, binder, chelating agent, oxygen scavenger, anti-wear agent, extreme pressure resistance additive, antimicrobial agent, pH adjuster, emulsifier, lubricity agent, vegetable oil, petroleum derived oil, high viscosity petroleum hydrocarbon oil, petroleum derivative, pour point depressant, moisture scavenger, defoamers, anti-misting agent, odorant, surfactant, humectant, rheology modifier, and colorant. 
     
     
         45 . The method of  claim 44 , wherein the antimicrobial agent selected from the group consisting of 1,2-Benzisothiazolin-3-one, sodium omadine, phenolics, p-chloro-m-cresol, halogen substituted carbamates, isothiazolone derivatives, bromonitriles dinitromorpholines, amphotericin, triazine, BIT, MIT, potassium sorbate, sodium benzoate, and glutaraldehyde. 
     
     
         46 . The method of  claim 44 , wherein the rheology modifier is selected from the group consisting of hydroxyethyl cellulose, carboxymethyl cellulose, xanthan gum, guar gum, starch, and polyanionic cellulose. 
     
     
         47 . The method of  claim 44 , wherein the microalgae is of the genus  Prototheca, Auxenochlorella, Chlorella , or  Parachlorella.    
     
     
         48 . The method of  claim 44 , wherein the microalgae is of the genus  Prototheca.    
     
     
         49 . The method of  claim 44 , wherein the microalgae is  Prototheca moriformis.    
     
     
         50 . A method for providing lubrication to a surface, the method comprising applying a lubricant to the surface, the lubricant comprising oleaginous microalgal biomass, wherein the oleaginous microalgal biomass is unlysed cells of the genus  Prototheca , wherein the oleaginous microalgal biomass comprises at least 20% lipid by dry cell weight, said lubricant further comprising at least one other ingredient selected from the group consisting of anti-oxidant, corrosion inhibitor, metal deactivator, binder, chelating agent, oxygen scavenger, anti-wear agent, extreme pressure resistance additive, antimicrobial agent, pH adjuster, emulsifier, lubricity agent, vegetable oil, petroleum derived oil, high viscosity petroleum hydrocarbon oil, petroleum derivative, pour point depressant, moisture scavenger, defoamers, anti-misting agent, odorant, surfactant, humectant, rheology modifier, and colorant. 
     
     
         51 . The method of claim  1 , wherein the oleaginous microalgal biomass is  Prorotheca moriformis.