Device for putting material into cell

Abstract

The present invention relates to a device for putting material into a cell to be modified. More particularly, the present invention is directed to a device formed within one solid material and comprises, a first passage on which the cell passes; a second passage on which the material passes and connected to the first passage at a position randomly selected between both ends of the first passage; and an apparatus which applies pressure difference or electric potential difference on the first passage and the second passage, and is also directed to a process for making the device.

What is claimed is: 
     
         1 . A process for forming a device within one solid for putting material into a cell, comprising:
 (i) a step of forming a first passage on which the cell passes within the one solid by irradiation of LASER;   (ii) a step of forming a second passage on which the material passes and connected to the first passage at a position randomly selected between both ends of the first passage, within the one solid by irradiation of LASER; and   (iii) installing an apparatus which applies electric potential difference between the first passage and the second passage.   
     
     
         2 . The process of  claim 1 , wherein the device comprises one or more of the second passage. 
     
     
         3 . The process of  claim 1 , wherein diameter of both ends of the first passage are larger than diameter of middle section of the first passage. 
     
     
         4 . The process of  claim 3 , wherein the inner diameter of both ends of the first passage is 10 μm to 200 μm. 
     
     
         5 . The process of  claim 4 , wherein the inner diameter of middle portion of the first passage is 3 μm to 150 μm. 
     
     
         6 . The process of  claim 5 , wherein the inner diameter of the second passage is 10 nm to 1,000 nm. 
     
     
         7 . The process of  claim 1 , wherein the electric potential difference between the first passage and the second passage is 0.5 V to 100 V. 
     
     
         8 . The process of  claim 7 , wherein the electric potential difference between the first passage and the second passage is 0.8 V to 50 V. 
     
     
         9 . The process of  claim 8 , wherein the electric potential difference between the first passage and the second passage is 1.0 V to 10 V. 
     
     
         10 . The process according to  claim 1 , the LASER is femto second laser. 
     
     
         11 . The process according to  claim 4 , the transparency of the solid is higher than 5%. 
     
     
         12 . The process according to  claim 11 , the solid is selected from the group consisting of glass, thermoplastic polymer and thermosetting polymer.