Herbal composition for healing of wound, cuts and burns and a method of synthesizing thereof

Abstract

The embodiments herein disclose a method for synthesizing an ointment from water plantain for the wounds, cuts and burnt tissues. The water plantain plant sample is collected and subjected for botanical analysis. The water plantain plant sample are pre-treated and dried. The dried water plantain plant samples are grinded to a powder. The water plantain plant powder is subjected to maceration/soaking for obtaining hydroalcoholic extract. A total of 5 litres of hydroalcoholic solvent extract comprising the essential nutrients and components of water plantain plant is obtained. A predetermined amount of the hydroalcoholic solvent extract is condensed in a rotary evaporator. The water plantain plant powder and hydroalcoholic solvent extract are tested for blood clotting. The hydroalcoholic solvent extract is mixed with Vaseline to obtain an ointment. The ointment is tested on rat wounds to find that the wound is healed in 15 days.

What is claimed is: 
     
         1 . A method for extracting the hydro alcoholic solvent extract comprising nutrients and components of water plantain plant for synthesizing an ointment for application on wounds, cuts and burnt tissues, the method comprises the steps of:
 pre-treating a plurality of water plantain plant samples;   cleaning and dis-infecting the plurality of water plantain plant samples;   drying the plurality of cleaned and disinfected water plantain plant samples in open air and in normal atmospheric conditions, and wherein the plurality of water plantain plant samples are dried in a shade area or dark room without exposing to any light source;   grinding the dried water plantain plant samples to obtain a powder using a grinding machine and a sieve;   subjecting the water plantain plant powder for maceration/soaking in a solvent for obtaining hydro alcoholic extract for synthesizing an ointment;   condensing a predetermined amount of hydro alcoholic solvent extract by heating in a water bath of a rotary evaporator for a predetermined time period, and wherein the hydro alcoholic solvent extract is heated to separate the solvent and to obtain a concentrated hydro alcoholic solvent extract;   cooling the concentrated hydro alcoholic solvent extract; and   analysing a plurality of nutrients and components present in the hydro alcoholic solvent extract obtained from water plantain by Gas chromatography-mass spectrometry (GC-MS) machine, and wherein the hydro alcoholic solvent extract obtained from the water plantain plant and the powder of the water plantain plant are analysed for blood clotting blood/blood coagulating properties.   
     
     
         2 . The method according to  claim 1 , wherein the step of pre-treating the water plantain plant sample comprises:
 collecting the water plantain plant samples of genus Alisma in the spring season;   drying the collected water plantain plant samples in open air, avoiding light and in normal atmospheric conditions; and   determining whether the collected water plantain plant samples belong to genus Alisma by testing the water plantain plant samples in a herbarium, and wherein the collected water plantain plant samples are confirmed to belong to genus Alisma.   
     
     
         3 . The method according to  claim 1 , wherein the step of subjecting the water plantain plant powder for maceration/soaking in a solvent for obtaining hydro alcoholic extract for synthesizing an ointment, comprises:
 adding a preset amount of the water plantain plant powder in percolator machine, and wherein the preset amount of the water plantain plant powder added to the percolator machine 677 grains;   adding preset amount of solvent in the percolator machine comprising water plantain plant powder, and wherein the solvent is 70% v/v ethanol, and wherein the preset amount of the solvent added to the percolator machine 3500 cc;   incubating the water plantain plant powder with solvent in percolator machine for a predetermined time period, and wherein the predetermined time period is 72 hours;   collecting a preset amount of the solvent extract after 72 hours by opening an outlet of the percolator machine, and wherein the preset amount of the solvent extract collected after 72 hours through the opening outlet of percolator machine is1800 cc;   adding a preset amount of solvent into the percolator machine comprising a mixture of solvent and water plantain plant powder, and wherein the solvent is the 70% v/v ethanol, and wherein the preset amount of solvent added to the percolator machine comprising a mixture of solvent and water plantain plant powder is 2000 cc;   incubating the solvent and the mixture of solvent and water plantain plant powder in percolator machine for a predetermined time period, and wherein the predetermined time period is 48 hours;   collecting a preset amount of the solvent extract after 48 hours by opening the outlet of percolator machine, and wherein the preset amount of solvent extract collected after 48 hours by opening the outlet of percolator machine is 1800 cc;   adding a preset amount of solvent into the percolator machine comprising a mixture of solvent and water plantain plant powder, and wherein the solvent is the 70% v/v ethanol, and wherein the preset amount of solvent added to the percolator machine is 2000 cc;   incubating the solvent and the mixture of solvent and water plantain plant powder in percolator machine for a predetermined period of time, and wherein the predetermined time period is 24 hours;   collecting a preset amount of the solvent extract after 24 hours by opening the outlet of percolator machine, and wherein the preset amount of the solvent extract collected after 24 hours by opening the outlet of percolator machine is1400 cc;   obtaining a preset total amount of hydro alcoholic solvent extract comprising the essential nutrients and natural components of water plantain plant, and wherein the total amount of a hydro alcoholic solvent extract total of 5 litres (1800 cc+1800 cc+1400 cc).   
     
     
         4 . The method according to  claim 1 , wherein the Gas chromatography-mass spectrometry (GC-MS) analysis of hydroalcoholic solvent extract obtained from the plurality of water plantain samples illustrates a presence of a plurality of nutrients and components, and wherein the plurality of nutrients and components are proteins, starch, resins, furfur aldehyde, asparagine, alkaloid, sugar, phytosterol, a plurality of fatty acids and a plurality of colour less solids. 
     
     
         5 . The method according to  claim 4 , wherein the plurality of fatty acids are palmitic acid, stearic acid, linoleic acid and oleic acid. 
     
     
         6 . The method according to  claim 4 , wherein the resin component in the hydro alcoholic solvent extract is a tissue regeneration agent, and wherein the resin provides nutrients required for a cell division and wherein the resin promotes a tissue healing process. 
     
     
         7 . The method according to  claim 1 , wherein hydro alcoholic solvent extract at a plurality of concentration levels are tested for blood clotting/blood coagulation property, and wherein the plurality of concentration levels of the hydro alcohol solvent extract are 25 mg/L (λ), 50 mg/L (λ), 75 mg/L (λ) and 100 mg/L (λ), and wherein the hydro alcohol solvent extract with a concentration of 25 mg/L (λ) illustrate a time period of 4 minute 15 seconds for clotting 2 cc of blood when compared to the hydro alcohol solvent extract with a mutually different concentration levels, and wherein the mutually different concentration levels are 50 mg/L (λ), 75 mg/L (λ) and 100 mg/L (λ). 
     
     
         8 . A composition for application on wounds, cuts and burnt tissues, the composition comprises:
 a predetermined amount of hydroalcoholic solvent extract, and wherein the hydroalcoholic solvent extract is obtained from the water plantain plant powder by a maceration/soaking method; and   a predetermined amount of a petroleum jelly, and wherein the petroleum jelly is Vaseline, and wherein a plurality of nutrients and components are present in the hydro alcoholic solvent extract obtained from water plantain plant.   
     
     
         9 . The composition according to  claim 8 , wherein the plurality of nutrients and components present in the hydro alcoholic solvent extract are a plurality of proteins, starch, resins, furfur aldehyde, asparagine, alkaloid, sugar, phytosterol, a plurality of fatty acids and a plurality of colour less solids. 
     
     
         10 . The composition according to  claim 9 , wherein the plurality of fatty acids are palmitic acid, stearic acid, linoleic acid and oleic acid. 
     
     
         11 . The composition according to  claim 9 , wherein the resin component in the hydro alcoholic solvent extract is a tissue regeneration agent, and wherein the resin provides nutrients required for a cell division and wherein the resin promotes tissue healing.