Hybridization probes and methods

Abstract

The present invention relates to compositions and methods for detection, analysis, and treatment of nucleic acids. In particular, the present invention relates to compositions and methods for generating and using hybridization probes.

1 - 20 . (canceled) 
     
     
         21 . A probe set generated by a method of generating a probe to a nucleic acid target of interest, comprising:
 a) identifying regions of said nucleic acid target of interest substantially free of undesired sequences that are at least 100 bp in length;   b) generating a plurality of probe-containing nucleic acids corresponding to said regions substantially free of undesired sequence; and   c) fragmenting said probe-containing nucleic acids to generate probes of said probe set.   
     
     
         22 . The probe set of  claim 21 , wherein said probes are hybridization probes. 
     
     
         23 . The probe set of  claim 21 , wherein said probes detect expression of an oncogene or chromosomal aneuploidy. 
     
     
         24 . The probe set of  claim 23 , wherein said oncogene is selected from Her2, p53, and p16. 
     
     
         25 . A method of performing a hybridization assay, comprising contacting a target nucleic acid with said probes of  claim 21 . 
     
     
         26 . The method of  claim 25 , wherein said hybridization assay is an ISH assay.