Method for manufacturing a microbial detection device, microbial detection method, microbial detection kit, and microbial detection device

Abstract

The present invention provides a method for manufacturing a microbial detection device, microbial detection method, microbial detection kit and microbial detection device. The manufacturing method includes the following steps: defining a sampling portion and a reaction portion on a substrate. Fiber materials are disposed in the reaction portion and a surface of the reaction portion which contacts with the fiber materials comprises abundant hydroxyl groups. Reaction reagents are then added into the fiber materials. An acidic solution is applied to treat the fiber materials and the hydroxyl groups in the reaction portion.

1 . A method for manufacturing a microbial detection device, including steps of:
 defining a sampling zone and a reacting zone on a substrate;   disposing a fiber material in the reacting zone wherein the surface of the reacting zone which contacts with the fiber material comprises abundant hydroxyl groups;   adding a reacting reagent onto the fiber material; and   applying an acidic solution to treat the fiber material and the hydroxyl groups.   
     
     
         2 . The method as claimed in  claim 1 , wherein the reacting reagent comprises at least one selected from a group consisting of 5-methylphenazinium methosulfate and diaphorase; and at least one selected from a group consisting of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride (INT), water-soluble tetrazolium salts (WSTs), and (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT). 
     
     
         3 . The method as claimed in  claim 1 , wherein the fiber material is α-cellulose particles. 
     
     
         4 . The method as claimed in  claim 1 , wherein the acidic solution contacts the surface of the reacting zone to treat those hydroxyl groups after the acidic solution penetrates through the fiber material. 
     
     
         5 . The method as claimed in  claim 1 , wherein the substrate is a fibrous substrate. 
     
     
         6 . The method as claimed in  claim 1 , wherein the reacting zone has an accommodating space, wherein the accommodating space is formed on a surface of the substrate and the fiber material is disposed in the accommodating space. 
     
     
         7 . A microbial detection kit, comprising:
 a detection device, including:   a substrate having a sampling zone and a reacting zone, wherein the reacting zone includes a fiber material and a reacting reagent which comprises at least one selected from a group consisting of 5-methylphenazinium methosulfate and diaphorase; and at least one selected from a group consisting of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride (INT), water-soluble tetrazolium salts (WSTs), and (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT); and   an acidic solution for treating the fiber material and the hydroxyl groups on a surface of the reacting zone which contact with the fiber material.   
     
     
         8 . The microbial detection kit claimed in  claim 7 , wherein the fiber material is α-cellulose particles. 
     
     
         9 . The microbial detection kit claimed in  claim 7 , wherein the fiber material is disposed on the reacting zone after treated by the acidic solution. 
     
     
         10 . A microbial detection device, comprising:
 a substrate having a sampling zone and a reacting zone, wherein the reacting zone includes:   a fiber material; and   a reacting reagent, wherein the reacting reagent comprises at least one selected from a group consisting of 5-methylphenazinium methosulfate and diaphorase; and at least one selected from a group consisting of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride (INT), water-soluble tetrazolium salts (WSTs), and (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT);   wherein the fiber material and hydroxyl groups of a surface of the reacting zone which contact with the fiber material are treated with an acidic solution.