Skin care applications of extracellular metabolites from bacillus coagulans

Abstract

Disclosed are the skin care applications of a composition containing the partially purified extracellular metabolite isolated from Bacillus coagulans MTCC 5856. More specifically, the invention discloses the ability of the partially purified extracellular metabolite isolated from Bacillus coagulans MTCC 5856 to confer protection to the skin fibroblasts against UV induced cell damage and apoptosis, oxidative stress and inflammation.

We claim: 
     
         1 . A method of protecting skin fibroblasts from UV induced damage, said method comprising steps of bringing into contact skin fibroblast cells with effective concentration of a composition containing partially purified extracellular metabolite preparation from  Bacillus coagulans , to bring about the effect of protection against UV-A and UV-B induced cell damage and apoptosis. 
     
     
         2 . The method as in  claim 1 , wherein the strain of  Bacillus coagulans  is  Bacillus coagulans  MTCC 5856. 
     
     
         3 . The method as in  claim 1 , wherein effective concentration of the partially purified extracellular metabolite preparation is 0.01% v/v to 2.0% v/v of the total composition. 
     
     
         4 . A composition containing partially purified extracellular metabolite preparation from  Bacillus coagulans  for use as a skin care agent, wherein the composition confers skin protection by a) protecting the skin against UV-A and UV-B induced cell damage and apoptosis, b) reducing oxidative stress in the skin by scavenging ROS and c) reducing inflammation by decreasing the levels of inflammatory markers in the skin. 
     
     
         5 . The composition as in  claim 4 , wherein the strain of  Bacillus coagulans  is  Bacillus coagulans  MTCC 5856. 
     
     
         6 . The composition as in  claim 4 , wherein effective concentration of the partially purified extracellular metabolite preparation is 0.01% v/v to 2.0% v/v of the total composition. 
     
     
         7 . The composition as in  claim 4 , wherein the composition is formulated with pharmaceutically/cosmeceutically acceptable excipients, adjuvants, bases, diluents, carriers, conditioning agents, bioavailability enhancers, antioxidants and preservatives and/or incorporated into formulations containing skin care ingredients and administered topically in the form of creams, gels, lotions, powder, serum, oil, suspensions, ointments, soaps, scrubs, emulsions, and compacts. 
     
     
         8 . A composition containing partially purified extracellular metabolite isolated from  Bacillus coagulans  for use as an anti-oxidant and anti-inflammatory agent. 
     
     
         9 . The composition as in  claim 8 , the strain of  Bacillus coagulans  is  Bacillus coagulans  MTCC 5856. 
     
     
         10 . The composition as in  claim 8 , wherein the composition is formulated with pharmaceutically/cosmeceutically acceptable excipients, adjuvants, bases, diluents, carriers, conditioning agents, bioavailability enhancers, and preservatives and/or incorporated into formulations containing skin care ingredients and administered topically in the form of creams, gels, lotions, powder, serum, oil, suspensions, ointments, soaps, scrubs, emulsions, and compacts.