US2018334658A1PendingUtilityA1

Methods and compositions for producing active vitamin k-dependent proteins

73
Assignee: UNIV NORTH CAROLINA CHAPEL HILLPriority: Mar 15, 2005Filed: Oct 26, 2017Published: Nov 22, 2018
Est. expiryMar 15, 2025(expired)· nominal 20-yr term from priority
Inventors:Darrel W. StaffordTao Li
C12N 9/64C12Q 2600/106C12Q 2600/156C12Q 2600/172C12Y 101/04001C12N 9/88C12N 9/0006C12Y 401/0109C12Q 2600/158C12Y 304/21006C12Q 1/6883C12N 9/50
73
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Claims

Abstract

The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.

Claims

exact text as granted — not AI-modified
That which is claimed is: 
     
         1 . A method of increasing the amount of carboxylated vitamin K dependent protein in a cell, comprising introducing into a cell that expresses a first nucleic acid encoding a vitamin K dependent protein a second heterologous nucleic acid encoding vitamin K epoxide reductase (VKOR) under conditions whereby said first and second nucleic acids are expressed to produce a vitamin K dependent protein and VKOR, respectively.

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