Cell culture medium and culture method using the same

Abstract

It is an object of the present invention to provide a cell culture medium capable of enhancing cell growth efficiency without using feeder cells, in particular which does not comprise serum. The present invention provides a cell culture medium which comprises growth arrest-specific 6 (GAS6) and does not comprise serum.

1 . A cell culture medium, which comprises growth arrest-specific 6 (GAS6), a basal medium, and at least one selected from the group consisting of decorin, matrix metalloproteinase-3 (MMP3), osteopontin (OPN), TNF-related weak inducer of apoptosis receptor (TWEAK R), insulin-like growth factor-binding protein 2 (IGFBP2), galectin 1 (LGALS1), and insulin-like growth factor-1 (IGF-1),
 wherein the cell culture medium does not comprise serum, and the concentration of the GAS6 contained in the cell culture medium is between 2 ng/ml and 100 ng/ml.   
     
     
         2 . The cell culture medium according to  claim 1 , wherein the cell culture medium supports the growth of iPS cells (induced pluripotent stem cells). 
     
     
         3 . The cell culture medium according to  claim 1 , wherein the basal medium is BME medium, BGJb medium, CMRL1066 medium, Glasgow MEM medium, Improved MEM Zinc Option medium, DMEM medium, Medium 199 medium, Eagle MEM medium, aMEM medium, DMEM medium, Ham medium, RPMI 1640 medium, Fischer's medium, or a mixed medium thereof. 
     
     
         4 . The cell culture medium according to  claim 1 , wherein the basal medium is CHO-S-SFM Hybridoma-SFM, eRDF Dry Powdered Media, UltraCULTURE™, UltraDOMA™, UltraCHO™, and UltraMDCK™, Essential 8, STEMPRO hESC SFM, mTeSR1, TeSR2, or ReproXF. 
     
     
         5 . The cell culture medium according to  claim 1 , comprising decorin, galectin 1 (LGALS1), and insulin-like growth factor-1 (IGF-1). 
     
     
         6 . The cell culture medium according to  claim 1 , excluding albumin. 
     
     
         7 . The cell culture medium according to  claim 1 , excluding a serum-derived component. 
     
     
         8 . The cell culture medium according to  claim 1 , which is a liquid cell culture medium. 
     
     
         9 . The cell culture medium according to  claim 2 , which is a liquid cell culture medium.