Solid Phase Assay for Detecting Biomarkers for Cardiodiabetes

Abstract

The invention provides compositions and methods for determining cardiodiabetes status in a subject. The invention also provides compositions and methods for treating a subject experiencing cardiodiabetes.

We claim: 
     
         1 . A composition comprising a solid support comprising:
 (a) a capture binding ligand selective for adiponectin,   (b) a capture binding ligand selective for high sensitivity C-reactive protein (hsCRP) wherein the capture binding ligand can detect a hsCRP concentration of less than 10 mg/L, and   (c) a capture binding ligand selective for intact proinsulin.   
     
     
         2 . The composition of  claim 1  wherein one of the capture binding ligands comprises an antibody. 
     
     
         3 . The composition of  claim 1  wherein the composition further comprises:
 (a) a soluble capture ligand selective for adiponectin, 
 (b) a soluble capture ligand selective for hsCRP, and 
 (c) a soluble capture ligand selective for intact proinsulin. 
 
     
     
         4 . The composition of  claim 3  wherein each of the soluble capture ligands comprises a detectable marker. 
     
     
         5 . The composition of  claim 4  wherein a detectable marker is a fluorophore. 
     
     
         6 . The composition of  claim 4  wherein a detectable marker is a conjugated enzyme. 
     
     
         7 . The composition of  claim 6  wherein the conjugated enzyme is horseradish peroxidase. 
     
     
         8 . The composition of  claim 1  further comprising a detector. 
     
     
         9 .- 22 . (canceled)