Compositions of bioactive fulvate fractions and uses thereof

Abstract

Compositions with fulvate fractions alone or in combination with growth factors, bioactive fragmented peptides, or combinations thereof are disclosed herein. Also disclosed are methods of using said compositions for the catalyzing cellular regeneration, including the healing, treatment, or prevention of skin disorders. Also disclosed are methods for extracting, isolating, and purifying fulvate fractions for use in the manufacture of said compositions.

What is claimed is: 
     
         1 . A method for extracting a bioactive polyelectrolyte from humified organic matter (HOM), wherein the bioactive polyelectrolyte comprises a fulvate fraction, the method comprising:
 providing an aqueous slurry comprising HOM;   applying the aqueous slurry to high pressure column fractionation to obtain fractionated samples;   applying the fractionated samples to molecular sieving; and   isolating a fulvate fraction.   
     
     
         2 . The method of  claim 1 , wherein the isolated fulvate fraction comprises one or more bioactive fragmented peptides. 
     
     
         3 . The method of  claim 2 , wherein the one or more bioactive fragmented peptide is a tigerinin-based peptide. 
     
     
         4 . The method of  claim 1 , wherein the isolated fulvate fraction has an approximate molecular formula of C 12 H 16 O 9 . 
     
     
         5 . The method of  claim 1 , wherein the fulvate fraction has an average molecular weight of 309 Da as measured by vapor pressure osmometry.